Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche

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dc.contributor.authorBurger, A. L.en_ZA
dc.contributor.authorBotha, F. C.en_ZA
dc.date.accessioned2013-01-23T14:11:11Z
dc.date.available2013-01-23T14:11:11Z
dc.date.issued2004
dc.descriptionCITATION: Burger, A. L. & Botha, F. C. 2004. Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche. Vitis, 43(2), doi:10.5073/vitis.2004.43.59-64.
dc.descriptionThe original publication is available at https://ojs.openagrar.de
dc.description.abstractThe gene expression patterns in ripening fruit of a high quality wine cultivar (Cabernet Sauvignon) and a poor quality wine cultivar (Clairette blanche) were studied using cDNA-AFLP fingerprinting. Total RNA from "immature" (14-weeks post flowering) and "mature" (18-weeks post flowering) berries were used to study ripening-related gene expression in post-véraison stages of berry development. A total of 1,276 fragments were analysed, of which 175 appeared to be ripening-related. Average pairwise differences of the fragments amplified from "immature" and "mature" Cabernet Sauvignon and Clairette blanche berries, revealed a high level of similarity between the two cultivars. 70 % of the ripening-related fragments were cultivar-specific. The number of cultivar-specific and/or ripening-related fragments amplified, depended on the selective nucleotides of the primers used in the cDNA-AFLP analysis. Reverse slot blot and northern blot analysis confirmed that the expression of the identified genes were ripening-related.
dc.description.urihttps://ojs.openagrar.de/index.php/VITIS/article/view/4350
dc.description.versionPublisher's version
dc.format.extent6 pages
dc.identifier.citationBurger, A. L. & Botha, F. C. 2004. Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche. Vitis, 43(2), doi:10.5073/vitis.2004.43.59-64.
dc.identifier.issn2367-4156 (online)
dc.identifier.urihttp://hdl.handle.net/10019.1/78646
dc.language.isoen
dc.publisherJulius Kühn-Institut
dc.rights.holderAuthor retains copyright
dc.subject.otherWine and wine makingen_ZA
dc.titleRipening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blancheen_ZA
dc.typeArticle
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