Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Julius Kühn-Institut
Abstract
The gene expression patterns in ripening fruit of a high quality wine cultivar (Cabernet Sauvignon) and a poor quality wine cultivar (Clairette blanche) were studied using cDNA-AFLP fingerprinting. Total RNA from "immature" (14-weeks post flowering) and "mature" (18-weeks post flowering) berries were used to study ripening-related gene expression in post-véraison stages of berry development. A total of 1,276 fragments were analysed, of which 175 appeared to be ripening-related. Average pairwise differences of the fragments amplified from "immature" and "mature" Cabernet Sauvignon and Clairette blanche berries, revealed a high level of similarity between the two cultivars. 70 % of the ripening-related fragments were cultivar-specific. The number of cultivar-specific and/or ripening-related fragments amplified, depended on the selective nucleotides of the primers used in the cDNA-AFLP analysis. Reverse slot blot and northern blot analysis confirmed that the expression of the identified genes were ripening-related.
Description
CITATION: Burger, A. L. & Botha, F. C. 2004. Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche. Vitis, 43(2), doi:10.5073/vitis.2004.43.59-64.
The original publication is available at https://ojs.openagrar.de
The original publication is available at https://ojs.openagrar.de
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Citation
Burger, A. L. & Botha, F. C. 2004. Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche. Vitis, 43(2), doi:10.5073/vitis.2004.43.59-64.