10Kin1day : a bottom-up neuroimaging initiative
Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media
Abstract
Parkinson’s disease (PD) is a well-known neurodegenerative disease with a strong
association established with systemic inflammation. Recently, the role of the gingipain
protease group from Porphyromonas gingivalis was implicated in Alzheimer’s disease
and here we present evidence, using a fluorescent antibody to detect gingipain
R1 (RgpA), of its presence in a PD population. To further elucidate the action
of this gingipain, as well as the action of the lipopolysaccharide (LPS) from
P. gingivalis, low concentrations of recombinant RgpA and LPS were added to
purified fluorescent fibrinogen. We also substantiate previous findings regarding PD by
emphasizing the presence of systemic inflammation via multiplex cytokine analysis, and
demonstrate hypercoagulation using thromboelastography (TEG), confocal and electron
microscopy. Biomarker analysis confirmed significantly increased levels of circulating
proinflammatory cytokines. In our PD and control blood analysis, our results show
increased hypercoagulation, the presence of amyloid formation in plasma, and profound
ultrastructural changes to platelets. Our laboratory analysis of purified fibrinogen with
added RgpA, and/or LPS, showed preliminary data with regards to the actions of
the protease and the bacterial membrane inflammagen on plasma proteins, to better
understand the nature of established PD.
Description
CITATION: Van Den Heuvel, M. P., et al. 2019. 10Kin1day : a bottom-up neuroimaging initiative. Frontiers in Neurology, 10:425, doi:10.3389/fneur.2019.00425.
The original publication is available at https://www.frontiersin.org
The original publication is available at https://www.frontiersin.org
Keywords
Brain -- Imaging, Magnetic resonance imaging, Diffusion magnetic resonance imaging, Brain mapping, Connectome analysis
Citation
Van Den Heuvel, M. P., et al. 2019. 10Kin1day : a bottom-up neuroimaging initiative. Frontiers in Neurology, 10:425, doi:10.3389/fneur.2019.00425