Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae
Date
1993
Authors
Southgate V.J.
Steyn A.J.C.
Pretorius I.S.
Van Vuuren H.J.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Replacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.
Description
Keywords
amylase, mating hormone alpha factor, signal peptide, bacillus amyloliquefaciens, enzyme assay, enzyme induction, enzyme release, enzyme synthesis, gene transfer, nonhuman, note, priority journal, promoter region, saccharomyces cerevisiae, alpha-Amylase, Bacillus, Base Sequence, Gene Expression Regulation, Enzymologic, Molecular Sequence Data, Peptides, Saccharomyces cerevisiae, Support, Non-U.S. Gov't, Transcription, Genetic, Transfection
Citation
Applied and Environmental Microbiology
59
4
59
4