Expression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae

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dc.contributor.authorSouthgate V.J.
dc.contributor.authorSteyn A.J.C.
dc.contributor.authorPretorius I.S.
dc.contributor.authorVan Vuuren H.J.J.
dc.date.accessioned2011-05-15T15:56:34Z
dc.date.available2011-05-15T15:56:34Z
dc.date.issued1993
dc.description.abstractReplacement of the regulatory and secretory signals of the α-amylase gene (AMY) from Bacillus amyloliquefaciens with the complete yeast pheromone α- factor prepro region (MFα1(P)) resulted in increased levels of extracellular α-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MFα1 spacer region (Lys-Arg-Glu-Ala-Glu- Ala) yielded decreased levels of extracellular α-amylase.
dc.description.versionNote
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation59
dc.identifier.citation4
dc.identifier.issn992240
dc.identifier.urihttp://hdl.handle.net/10019.1/9925
dc.subjectamylase
dc.subjectmating hormone alpha factor
dc.subjectsignal peptide
dc.subjectbacillus amyloliquefaciens
dc.subjectenzyme assay
dc.subjectenzyme induction
dc.subjectenzyme release
dc.subjectenzyme synthesis
dc.subjectgene transfer
dc.subjectnonhuman
dc.subjectnote
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectsaccharomyces cerevisiae
dc.subjectalpha-Amylase
dc.subjectBacillus
dc.subjectBase Sequence
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectMolecular Sequence Data
dc.subjectPeptides
dc.subjectSaccharomyces cerevisiae
dc.subjectSupport, Non-U.S. Gov't
dc.subjectTranscription, Genetic
dc.subjectTransfection
dc.titleExpression and secretion of Bacillus amyloliquefaciens α-amylase by using the yeast pheromone α-factor promoter and leader sequence in Saccharomyces cerevisiae
dc.typeNote
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