Department of Biochemistry
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Investigation of protein complexes involved in coenzyme A biosynthesis
(Stellenbosch : Stellenbosch University, 2022-12)ENGLISH ABSTRACT: The biochemistry of the CoA biosynthetic pathway has been extensively studied and the differences in how the pathway functions between domains of life has led to its validation in recent years as target ... -
Modular control analysis and its application to glucose metabolism in plasmodium falciparum-infected erythrocytes
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Disease treatment is achieved through the administration of medication acting at the molecular level which results in changes at the physiological level. These changes are caused by molecular interactions ... -
The role of a positive feedforward loop in regulating yeast glycolytic intermediate dynamics
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Yeast glycolysis is a well-studied model for metabolic pathways and fundamental aspects of regulation. Whereas negative feedback is common in metabolic networks; positive feedforward regulation is much ... -
Phasor representation of oscillatory biochemical pathways
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Oscillations or rhythms arise in many biological systems such as biochemical pathways. Numerous mathematical models and experimental data sets of such systems display sustained oscillations in both the ... -
On the relationship between thermodynamic disequilibrium ratio and flux control of metabolic pathways
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: This study presents a methodology to determine and compare flux control co- efficients, as defined in the metabolic control analysis framework, with dise- quilibrium ratios of reactions in metabolic ... -
Investigating the effects of treatment in HIV disease models
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: HIV/AIDS and disease response to antiretroviral (ARV ) drugs are of major importance to the developing world, and the disease remains a burden on society, as viral replication still needs to be ... -
Examining plasminogen activator-inhibitor-1 and C-reactive protein regulation by glucocorticoids and pro-inflammatory cytokines in liver cell lines
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Current knowledge suggests that a continued elevated acute phase response (APR), which is often referred to as low-grade inflammation, occurs during insulin resistance. The levels of acute phase ... -
Quantifying the insulin response in mouse C2C12 skeletal muscle: a minimal modelling approach
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: The insulin signalling cascade is one of the most important regulatory and signalling pathways in humans. Dysregulation or dysfunction of the insulin signalling path- ways often underlies the molecular ... -
The carbon switch at the level of pyruvate and phosphoenolpyruvate in sulfolobus solfataricus P2
(Frontiers Media, 2019-04-12)Sulfolobus solfataricus P2 grows on different carbohydrates as well as alcohols, peptides and amino acids. Carbohydrates such as D-glucose or D-galactose are degraded via the modified, branched Entner–Doudoroff (ED) pathway ... -
Differential modulation of gene expression encoding hepatic and renal xenobiotic metabolizing enzymes by an aspalathin-enriched rooibos extract and aspalathin
(Thieme Gruppe, 2019)Modulation of the expression of hepatic and renal genes encoding xenobiotic metabolizing enzymes by an aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was investigated in the liver and kidneys of F344 ... -
Degradation of proteins and starch by combined immobilization of protease, α-amylase and β-galactosidase on a single electrospun nanofibrous membrane
(MDPI, 2019-01-31)Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their ... -
Steroid metabolome analysis in disorders of adrenal steroid biosynthesis and metabolism
(Endocrine Society, 2019-07-11)Steroid biosynthesis and metabolism are reflected by the serum steroid metabolome and, in even more detail, by the 24-hour urine steroid metabolome, which can provide unique insights into alterations of steroid flow and ... -
Blood ketone bodies and breath acetone analysis and their correlations in type 2 diabetes mellitus
(MDPI, 2019-12-17)Analysis of volatile organic compounds in the breath for disease detection and monitoring has gained momentum and clinical significance due to its rapid test results and non-invasiveness, especially for diabetes mellitus ... -
Examining the transcription factor FOXO1 and its regulation by glucocorticoids and inflammatory mediators in hepatic insulin signalling
(Stellenbosch : Stellenbosch University, 2021-12)ENGLISH ABSTRACT: Insulin resistance is the decrease in responsiveness to normal circulating insulin levels, resulting in an attenuated biological response in target tissues (including the liver, muscle, ... -
Harmonizing semantic annotations for computational models in biology
(Oxford University Press, 2019)Life science researchers use computational models to articulate and test hypotheses about the behavior of biological systems. Semantic annotation is a critical component for enhancing the interoperability and reusability ... -
Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes : a comprehensive review
(Elsevier, 2019-07-27)Advances in technology have allowed for the sensitive, specific, and simultaneous quantitative profiling of steroid precursors, bioactive steroids and inactive metabolites, facilitating comprehensive characterization of ... -
Quantitation of the effects of cellular glucocorticoid receptor concentration on dimerization
(Stellenbosch : Stellenbosch University, 2021-04)ENGLISH ABSTRACT: The glucocorticoids (GCs) are a group of steroids affecting virtually every cell in the body. The ability of the GCs to modulate the effects of the immune system is of particular interest ... -
The development of standardized ion mobility and mass spectrometry metabolomics methods for the characterization of plant phenolics
(Stellenbosch : Stellenbosch University, 2021-04)ENGLISH ABSTRACT: Plants and plant-derived products contain a plethora of phenolic compounds, with a broad range of health benefits and useful applications such as in drug design. These phenolic compounds ... -
Vitamin in the crosshairs : targeting pantothenate and coenzyme a biosynthesis for new antituberculosis agents
(Frontiers Media, 2020)ENGLISH ABSTRACT: Despite decades of dedicated research, there remains a dire need for new drugs against tuberculosis (TB). Current therapies are generations old and problematic. Resistance to these existing therapies ... -
Antibody production against Staphylococcus aureus CoA biosynthesis enzymes and their application in protein level quantification
(Stellenbosch : Stellenbosch University, 2021-04)ENGLISH ABSTRACT: Antimicrobial resistance has become an increased burden worldwide as more and more human pathogens are becoming resistant to current antimicrobials. Therefore, the identification of novel drug targets and ...
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