Department of Biochemistry
Collections in this community
Recent Submissions
-
Filovirus disease outbreaks : a chronological overview
(SAGE, 2019)Filoviruses cause outbreaks which lead to high fatality in humans and non-human primates, thus tagging them as major threats to public health and species conservation. In this review, we give account of index cases responsible ... -
Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function
(Nature Research, 2018-10-02)S. cerevisiae Hal3 (ScHal3) is a moonlighting protein that, is in its monomeric state, regulates the Ser/Thr protein phosphatase Ppz1, but also joins ScCab3 (and in some instances the Hal3 paralog Vhs3) to form an unusual ... -
Effect of yeasts and oenological parameters on acetaldehyde production during alcoholic fermentation of South African grape musts
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: Acetaldehyde plays a role in the rate of fermentation and the quality of wine. High levels of acetaldehyde in fermenting juice may result in sluggish/stuck fermentations, and in wine, it may impart ... -
An investigation into the effects of macromolecular crowding on the kinetics of upper glycolytic enzymes in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: In order for mathematical models of metabolism to accurately emulate experimental data the conditions in which parameter values are obtained must be close to the actual in vivo environment. However, this ... -
Whole-body modelling of glucose and lactate dynamics in plasmodium falciparum malaria
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: Malaria is the most important parasitic disease affecting man, and continues to threaten public health in developing countries where the disease is endemic. Within the genus Plasmodium, falciparum is ... -
Investigating the dynamics of hydrogen peroxide metabolism in the peroxiredoxin system of Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: Reactive oxygen species (ROS) are derivations of molecular oxygen that can have detrimental e ects in cells. ROS can readily react with DNA, proteins and lipids often resulting in the loss of structure ... -
Identification and genomic distribution of select combinatorial post-translational modification patterns of Trypanosoma brucei histones
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT:Trypanosoma brucei is a dixenous parasitic protozoan, and the causative agent of African Sleeping sickness, or Human African Trypanosomiasis. Trypanosomes diverged very early on from the main eukaryotic ... -
Floral color, anthocyanin synthesis gene expression and control in Cape Erica species
(Frontiers Media, 2019-11-28)Introduction: The Cape Floristic Region (CFR) is a biodiversity hotspot, recognized globally for its unusually high levels of endemism. The origins of this biodiversity are a long-standing topic of research. The largest ... -
Leaps and bounds : geographical and ecological distance constrained the colonisation of the Afrotemperate by Erica
(BMC (part of Springer Nature), 2019-12-05)Background: The coincidence of long distance dispersal (LDD) and biome shift is assumed to be the result of a multifaceted interplay between geographical distance and ecological suitability of source and sink areas. Here, ... -
Strategies and opportunities for promoting bioinformatics in Zimbabwe
(Public Library of Science, 2018-11-29)Introduction: The increasing applications of advanced technologies in life sciences are fueling the growth of data from genome sequencing, functional genomics experiments, and macromolecular structure determination. ... -
Falsely elevated plasma testosterone concentrations in neonates : importance of LC-MS/ MS measurements
(De Gruyter, 2018-01)In newborns with atypical genitalia, suspicious for a disorder of sex development (DSD), measurement of testosterone is an essential part in the diagnostic workup. Previously, direct testosterone immunoassays have proven ... -
The multifaceted antibacterial mechanisms of the pioneering peptide antibiotics tyrocidine and gramicidin S
(American Society for Microbiology, 2018-10-09)ENGLISH ABSTRACT: Cyclic β-sheet decapeptides from the tyrocidine group and the homologous gramicidin S were the first commercially used antibiotics, yet it remains unclear exactly how they kill bacteria. We investigated ... -
Glycolytic flux control of glyceraldehyde 3-phosphate dehydrogenase in yeast
(Stellenbosch : Stellenbosch University, 2019-12)ENGLISH ABSTRACT: To save precious experimental time and resources and to gain a deeper understanding of living systems, modelling approaches and systems biological tools like Metabolic Control Analysis (MCA) offer the ... -
Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
(Public Library of Science, 2018-04-03)The malaria-causing blood stage of Plasmodium falciparum requires extracellular pantothenate for proliferation. The parasite converts pantothenate into coenzyme A (CoA) via five enzymes, the first being a pantothenate ... -
IMA Genome-F 11: Draft genome sequences of Fusarium xylarioides, Teratosphaeria gauchensis and T. zuluensis and genome annotation for Ceratocystis fimbriata
(BMC (part of Springer Nature), 2019-09-13)ENGLISH ABSTRACT: Draft genomes of the fungal species Fusarium xylarioides, Teratosphaeria gauchensis and T. zuluensis are presented. In addition an annotation of the genome of Ceratocystis fimbriata is presented. Overall ... -
Disease- and treatment-associated acquired glucocorticoid resistance
(BioScientifica, 2018-10-11)ENGLISH ABSTRACT: The development of resistance to glucocorticoids (GCs) in therapeutic regimens poses a major threat. Generally, GC resistance is congenital or acquired over time as a result of disease progression, prolonged ... -
Data comparing the separation and elution of vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometer (UPSFC-MS/MS) compared to liquid chromatography (LC) and data presenting approaches to UPSFC method optimization
(Elsevier, 2018)The data presented is related to the research article "Analysis of multiple vitamin D metabolites by ultra performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS)" (Jenkinson et al., 2018) ... -
Workflow for data analysis in experimental and computational systems biology : using Python as glue
(MDPI, 2019-07-18)ENGLISH ABSTRACT: Bottom-up systems biology entails the construction of kinetic models of cellular pathways by collecting kinetic information on the pathway components (e.g., enzymes) and collating this into a kinetic ... -
GR dimerization and the impact of GR dimerization on GR protein stability and half-life
(Frontiers Media, 2019-07-17)Pharmacologically, glucocorticoids, which mediate their effects via the glucocorticoid receptor (GR), are a most effective therapy for inflammatory diseases despite the fact that chronic use causes side-effects and ... -
Patterns of variation and chemosystematic significance of phenolic compounds in the genus cyclopia (fabaceae, podalyrieae)
(MDPI, 2019)ENGLISH ABSTRACT: As a contribution towards a better understanding of phenolic variation in the genus Cyclopia (honeybush tea), a collection of 82 samples from 15 of the 23 known species was analysed using liquid-chromat ...
RSS 1.0