Department of Biochemistry
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The carbon switch at the level of pyruvate and phosphoenolpyruvate in sulfolobus solfataricus P2
(Frontiers Media, 2019-04-12)Sulfolobus solfataricus P2 grows on different carbohydrates as well as alcohols, peptides and amino acids. Carbohydrates such as D-glucose or D-galactose are degraded via the modified, branched Entner–Doudoroff (ED) pathway ... -
Differential modulation of gene expression encoding hepatic and renal xenobiotic metabolizing enzymes by an aspalathin-enriched rooibos extract and aspalathin
(Thieme Gruppe, 2019)Modulation of the expression of hepatic and renal genes encoding xenobiotic metabolizing enzymes by an aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was investigated in the liver and kidneys of F344 ... -
Degradation of proteins and starch by combined immobilization of protease, α-amylase and β-galactosidase on a single electrospun nanofibrous membrane
(MDPI, 2019-01-31)Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their ... -
Steroid metabolome analysis in disorders of adrenal steroid biosynthesis and metabolism
(Endocrine Society, 2019-07-11)Steroid biosynthesis and metabolism are reflected by the serum steroid metabolome and, in even more detail, by the 24-hour urine steroid metabolome, which can provide unique insights into alterations of steroid flow and ... -
Harmonizing semantic annotations for computational models in biology
(Oxford University Press, 2019)Life science researchers use computational models to articulate and test hypotheses about the behavior of biological systems. Semantic annotation is a critical component for enhancing the interoperability and reusability ... -
Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes : a comprehensive review
(Elsevier, 2019-07-27)Advances in technology have allowed for the sensitive, specific, and simultaneous quantitative profiling of steroid precursors, bioactive steroids and inactive metabolites, facilitating comprehensive characterization of ... -
Vitamin in the crosshairs : targeting pantothenate and coenzyme a biosynthesis for new antituberculosis agents
(Frontiers Media, 2020)ENGLISH ABSTRACT: Despite decades of dedicated research, there remains a dire need for new drugs against tuberculosis (TB). Current therapies are generations old and problematic. Resistance to these existing therapies ... -
Profiling the production of antimicrobial secondary metabolites by xenorhabdus khoisanae J194 under different culturing conditions
(Frontiers Media, 2020-03-30)Species from the genus Xenorhabdus, endosymbiotic bacteria of Steinernema nematodes, produce several antibacterial and antifungal compounds, some of which are anti-parasitic. In this study, we report on the effect growth ... -
Characterisation and formulation of natural cyclodecapeptides with anti-Candida activity
(Stellenbosch : Stellenbosch University, 2021-03)ENGLISH ABSTRACT: The increase in the number of fungal infections since the 1980s is a major concern globally. Fungal infections affect various medical and agricultural sectors. A limited number of available antifungal ... -
Mathematical modelling of glycolysis in cancer cell lines
(Stellenbosch : Stellenbosch University, 2021-03)ENGLISH ABSTRACT: Most cancer cells exhibit an increase in glucose uptake and lactate produc- tion, a phenomenon known as the "Warburg effect" or aerobic glycolysis, and considered to be a hallmark of cancer ... -
Mathematical modelling of glycolysis in differentiated mouse adipocytes 3T3-L1
(Stellenbosch : Stellenbosch UniversityStellenbosch : Stellenbosch University, 2021-03)ENGLISH ABSTRACT: Diabetes mellitus is a metabolic disease characterised by high blood sugar levels, primarily caused by insulin resistance and the resulting inability of the body to process glucose. Any tissue or cell ... -
Modelling the insulin signaling pathway and glucose transport induction in differentiated 3T3-L1 mouse adipocytes
(Stellenbosch : Stellenbosch University, 2021-04)ENGLISH ABSTRACT: Insulin is the principal controller of energy homeostasis in the human body. It lowers postprandial blood glucose and enforces a change in cellu- lar metabolism to maintain glucose ... -
A bioinformatics tool to detect physical gene clusters in functional genomics data
(Stellenbosch : Stellenbosch University, 2021-03)ENGLISH ABSTRACT: Many studies have investigated the biological effects of the external environment on the genetic expression of a living cell. This particular study looks at gene clusters that are switched on at a series ... -
Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach
(MDPI, 2019-09-10)Enzymatic detoxification has become a promising approach for control of mycotoxins postharvest in grains through modification of chemical structures determining their toxicity. In the present study fumonisin esterase FumD ... -
Filovirus disease outbreaks : a chronological overview
(SAGE, 2019)Filoviruses cause outbreaks which lead to high fatality in humans and non-human primates, thus tagging them as major threats to public health and species conservation. In this review, we give account of index cases responsible ... -
An investigation into the biosynthesis and metabolism of adrenal C11-oxy steroids
(2020-12)ENGLISH ABSTRACT: This study describes: ▪ an in vitro investigation into the biosynthesis and metabolism of 11β-hydroxyprogesterone (11OHP4) and 21-deoxycortisol (21dF) by steroidogenic enzymes which ... -
Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function
(Nature Research, 2018-10-02)S. cerevisiae Hal3 (ScHal3) is a moonlighting protein that, is in its monomeric state, regulates the Ser/Thr protein phosphatase Ppz1, but also joins ScCab3 (and in some instances the Hal3 paralog Vhs3) to form an unusual ... -
The tyrocidines in the creation of antimicrobial cellulose and sterilizing materials
(Stellenbosch : Stellenbosch University, 2020-04)ENGLISH ABSTRACT: The rising resistance of pathogenic bacteria is of great concern, especially since resistance has been reported for five of the six common hospital acquired infections. Furthermore, continual ... -
Effect of yeasts and oenological parameters on acetaldehyde production during alcoholic fermentation of South African grape musts
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: Acetaldehyde plays a role in the rate of fermentation and the quality of wine. High levels of acetaldehyde in fermenting juice may result in sluggish/stuck fermentations, and in wine, it may impart ... -
An investigation into the effects of macromolecular crowding on the kinetics of upper glycolytic enzymes in Saccharomyces cerevisiae
(Stellenbosch : Stellenbosch University, 2020-03)ENGLISH ABSTRACT: In order for mathematical models of metabolism to accurately emulate experimental data the conditions in which parameter values are obtained must be close to the actual in vivo environment. However, this ...
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