Complexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells
Date
1989
Authors
Hanekom C.
Nel A.
Gittinger C.
Rheeder A.
Landreth G.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Treatment of Jurkat T-cells with anti-CD-3 monoclonal antibodies resulted in the rapid and transient activation of a serine kinase with utilized the microtubule-associated protein, MAP-2, as a substrate in vitro. The kinase was also activated on treatment of Jurkat cells with phytohaemagglutinin, but with a different time course. The activation of the MAP-2 kinase by anti-CD-3 antibodies was dose-dependent, with maximal activity observed at concentrations of > 500 ng/ml. Normal human E-rosette-positive T-cells also exhibited induction of MAP-2 kinase activity during anti-CD-3 treatment. The enzyme was optimally active in the presence of 2 mM-Mn2+; lower levels of activity were observed with Mg2+, even at concentrations up to 20 mM. The kinase was partially purified by passage over DE-52 Sephacel with the activity eluting as a single peak of 0.25 M-NaCl. The molecular mass was estimated to be 45 kDa by gel filtration. The activation of the MAP-2 kinase was probably due to phosphorylation of this enzyme as treatment with alkaline phosphatase diminished its activity. These data demonstrate that the stimulation of T-cells through the CD-3 complex results in the activation of a novel serine kinase which may be critically involved in signal transduction in these cells.
Description
Keywords
cd3 antigen, microtubule associated protein 2, monoclonal antibody, protein serine kinase, t lymphocyte receptor, cell culture, enzyme activation, leukemia cell line, priority journal, signal transduction, t lymphocyte, Antibodies, Monoclonal, Antigens, CD3, Antigens, Differentiation, T-Lymphocyte, Ca(2+)-Calmodulin Dependent Protein Kinase, Cell Line, Dose-Response Relationship, Immunologic, Enzyme Activation, Enzyme Induction, Human, Membrane Glycoproteins, Protein Kinases, Receptors, Antigen, T-Cell, Support, Non-U.S. Gov't, Support, U.S. Gov't, Non-P.H.S., Support, U.S. Gov't, P.H.S., T-Lymphocytes
Citation
Biochemical Journal
262
2
262
2