Complexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells

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dc.contributor.authorHanekom C.
dc.contributor.authorNel A.
dc.contributor.authorGittinger C.
dc.contributor.authorRheeder A.
dc.contributor.authorLandreth G.
dc.date.accessioned2011-05-15T16:03:24Z
dc.date.available2011-05-15T16:03:24Z
dc.date.issued1989
dc.description.abstractTreatment of Jurkat T-cells with anti-CD-3 monoclonal antibodies resulted in the rapid and transient activation of a serine kinase with utilized the microtubule-associated protein, MAP-2, as a substrate in vitro. The kinase was also activated on treatment of Jurkat cells with phytohaemagglutinin, but with a different time course. The activation of the MAP-2 kinase by anti-CD-3 antibodies was dose-dependent, with maximal activity observed at concentrations of > 500 ng/ml. Normal human E-rosette-positive T-cells also exhibited induction of MAP-2 kinase activity during anti-CD-3 treatment. The enzyme was optimally active in the presence of 2 mM-Mn2+; lower levels of activity were observed with Mg2+, even at concentrations up to 20 mM. The kinase was partially purified by passage over DE-52 Sephacel with the activity eluting as a single peak of 0.25 M-NaCl. The molecular mass was estimated to be 45 kDa by gel filtration. The activation of the MAP-2 kinase was probably due to phosphorylation of this enzyme as treatment with alkaline phosphatase diminished its activity. These data demonstrate that the stimulation of T-cells through the CD-3 complex results in the activation of a novel serine kinase which may be critically involved in signal transduction in these cells.
dc.description.versionArticle
dc.identifier.citationBiochemical Journal
dc.identifier.citation262
dc.identifier.citation2
dc.identifier.issn2646021
dc.identifier.urihttp://hdl.handle.net/10019.1/12606
dc.subjectcd3 antigen
dc.subjectmicrotubule associated protein 2
dc.subjectmonoclonal antibody
dc.subjectprotein serine kinase
dc.subjectt lymphocyte receptor
dc.subjectcell culture
dc.subjectenzyme activation
dc.subjectleukemia cell line
dc.subjectpriority journal
dc.subjectsignal transduction
dc.subjectt lymphocyte
dc.subjectAntibodies, Monoclonal
dc.subjectAntigens, CD3
dc.subjectAntigens, Differentiation, T-Lymphocyte
dc.subjectCa(2+)-Calmodulin Dependent Protein Kinase
dc.subjectCell Line
dc.subjectDose-Response Relationship, Immunologic
dc.subjectEnzyme Activation
dc.subjectEnzyme Induction
dc.subjectHuman
dc.subjectMembrane Glycoproteins
dc.subjectProtein Kinases
dc.subjectReceptors, Antigen, T-Cell
dc.subjectSupport, Non-U.S. Gov't
dc.subjectSupport, U.S. Gov't, Non-P.H.S.
dc.subjectSupport, U.S. Gov't, P.H.S.
dc.subjectT-Lymphocytes
dc.titleComplexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells
dc.typeArticle
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