Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion
Date
1999
Authors
Carroll N.M.
Adamson P.
Okhravi N.
Journal Title
Journal ISSN
Volume Title
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Abstract
The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which uses primers based on the 16S rRNA genes. The method was used prior to a PCR that amplified 10 fg of bacterial DNA. This method can be readily adapted to suit other sensitive PCRs required for clinical applications.
Description
Keywords
bacterial DNA, restriction endonuclease, ribosome RNA, taq polymerase, animal cell, article, DNA template, gene amplification, laboratory diagnosis, nonhuman, polymerase chain reaction, priority journal, protein analysis, DNA, Bacterial, Polymerase Chain Reaction, Taq Polymerase, Animalia, Bacteria (microorganisms)
Citation
Journal of Clinical Microbiology
37
10
37
10