Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion

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dc.contributor.authorCarroll N.M.
dc.contributor.authorAdamson P.
dc.contributor.authorOkhravi N.
dc.date.accessioned2011-05-15T15:56:58Z
dc.date.available2011-05-15T15:56:58Z
dc.date.issued1999
dc.description.abstractThe incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which uses primers based on the 16S rRNA genes. The method was used prior to a PCR that amplified 10 fg of bacterial DNA. This method can be readily adapted to suit other sensitive PCRs required for clinical applications.
dc.description.versionArticle
dc.identifier.citationJournal of Clinical Microbiology
dc.identifier.citation37
dc.identifier.citation10
dc.identifier.issn951137
dc.identifier.urihttp://hdl.handle.net/10019.1/10141
dc.subjectbacterial DNA
dc.subjectrestriction endonuclease
dc.subjectribosome RNA
dc.subjecttaq polymerase
dc.subjectanimal cell
dc.subjectarticle
dc.subjectDNA template
dc.subjectgene amplification
dc.subjectlaboratory diagnosis
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectprotein analysis
dc.subjectDNA, Bacterial
dc.subjectPolymerase Chain Reaction
dc.subjectTaq Polymerase
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.titleElimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion
dc.typeArticle
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