Screening of commercial hydrolases for the degradation of Ochratoxin A
Date
2000
Authors
Stander M.A.
Bornscheuer U.T.
Henke E.
Steyn P.S.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Ochratoxin A (OTA) is a nephrotoxic and carcinogenic mycotoxin. The toxin is a common contaminant of various foods and feeds and poses a serious threat to the health of both humans and animals. A number of commercial hydrolases were screened for the ability to degrade OTA to nontoxic compounds. A crude lipase from Aspergillus niger (Amano A) proved to substantially hydrolyze OTA to the nontoxic OTα and phenylalanine, as confirmed by HPLC with fluorescence detection. The enzyme was purified by anion exchange chromatography to homogeneity. Activity staining of the purified enzyme with α-naphthyl acetate/Fast Red revealed only one band exhibiting hydrolytic activity. The specific activity of the purified enzyme toward OTA was 2.32 units/mg.
Description
Keywords
carcinogen, esterase, hydrolase, mycotoxin, ochratoxin, triacylglycerol lipase, article, Aspergillus niger, carcinogenicity, chemical structure, enzyme activity, high performance liquid chromatography, kinetics, nephrotoxicity, nonhuman, Pseudomonas, toxin analysis, wheat, yeast, Aspergillus niger, Carcinogens, Esterases, Hydrolases, Kinetics, Lipase, Mycotoxins, Ochratoxins, Pseudomonas, Yeasts, Animalia, Aspergillus niger, Pseudomonas, Triticum aestivum
Citation
Journal of Agricultural and Food Chemistry
48
11
48
11