Screening of commercial hydrolases for the degradation of Ochratoxin A

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dc.contributor.authorStander M.A.
dc.contributor.authorBornscheuer U.T.
dc.contributor.authorHenke E.
dc.contributor.authorSteyn P.S.
dc.date.accessioned2011-05-15T15:56:53Z
dc.date.available2011-05-15T15:56:53Z
dc.date.issued2000
dc.description.abstractOchratoxin A (OTA) is a nephrotoxic and carcinogenic mycotoxin. The toxin is a common contaminant of various foods and feeds and poses a serious threat to the health of both humans and animals. A number of commercial hydrolases were screened for the ability to degrade OTA to nontoxic compounds. A crude lipase from Aspergillus niger (Amano A) proved to substantially hydrolyze OTA to the nontoxic OTα and phenylalanine, as confirmed by HPLC with fluorescence detection. The enzyme was purified by anion exchange chromatography to homogeneity. Activity staining of the purified enzyme with α-naphthyl acetate/Fast Red revealed only one band exhibiting hydrolytic activity. The specific activity of the purified enzyme toward OTA was 2.32 units/mg.
dc.description.versionArticle
dc.identifier.citationJournal of Agricultural and Food Chemistry
dc.identifier.citation48
dc.identifier.citation11
dc.identifier.issn218561
dc.identifier.other10.1021/jf000413j
dc.identifier.urihttp://hdl.handle.net/10019.1/10093
dc.subjectcarcinogen
dc.subjectesterase
dc.subjecthydrolase
dc.subjectmycotoxin
dc.subjectochratoxin
dc.subjecttriacylglycerol lipase
dc.subjectarticle
dc.subjectAspergillus niger
dc.subjectcarcinogenicity
dc.subjectchemical structure
dc.subjectenzyme activity
dc.subjecthigh performance liquid chromatography
dc.subjectkinetics
dc.subjectnephrotoxicity
dc.subjectnonhuman
dc.subjectPseudomonas
dc.subjecttoxin analysis
dc.subjectwheat
dc.subjectyeast
dc.subjectAspergillus niger
dc.subjectCarcinogens
dc.subjectEsterases
dc.subjectHydrolases
dc.subjectKinetics
dc.subjectLipase
dc.subjectMycotoxins
dc.subjectOchratoxins
dc.subjectPseudomonas
dc.subjectYeasts
dc.subjectAnimalia
dc.subjectAspergillus niger
dc.subjectPseudomonas
dc.subjectTriticum aestivum
dc.titleScreening of commercial hydrolases for the degradation of Ochratoxin A
dc.typeArticle
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