Doctoral Degrees (Chemical Pathology)

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    HbA1c as a screening tool for diabetes mellitus and its use with traditional and novel biochemical parameters to predict cardiovascular risk in a local urban community
    (Stellenbosch : Stellenbosh University, 2016-12) Zemlin, Annalise E.; Erasmus, Rajiv T.; Matsha, Tandi E.; Kengne, Andre P.; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Chemical Pathology.
    ENGLISH SUMMARY: Introduction The global obesity pandemic has reached Africa and the diabetes mellitus (DM) prevalence is increasing in parallel. A high prevalence of DM and risk for cardiovascular disease (CVD) has been described in the South African mixed ancestry population. Recent guidelines advocate using HbA1c as a diagnostic tool for DM and prediabetes, which is more convenient. However, various studies have challenged these cut-offs. There is a paucity of studies validating these cut-offs in Africa. As DM is considered a CVD risk equivalent, emerging markers of CVD and adiposity also need evaluation. The adipokine adiponectin has anti-diabetic, anti-atherogenic and anti-inflammatory properties and levels decrease in obesity. E-selectin, a marker of endothelial cell dysfunction, is associated with subclinical atherosclerosis and hyperglycaemia. Carotid intima-media thickness (CIMT) is a noninvasive measure of subclinical atherosclerosis. The aim of this investigation was to verify recommended HbA1c cut-offs to diagnose DM and prediabetes and to examine the usefulness of emerging markers of subclinical CVD in our population. Methods This investigation consists of four substudies and was performed on participants of the Bellville South Africa Study. In the first, we challenged the recommended HbA1c cut-off of 6.5% to diagnose DM in 946 participants using oral glucose tolerance test (OGTT), fasting blood glucose (FBG), and receiver operator characteristic (ROC) curves. In the second, we derived an optimal HbA1c cut-off to detect prediabetes in 667 participants and validated this in two populations, using OGTT and ROC curves. In the third, we determined high molecular weight (hmw)-adiponectin levels in 101 participants, compared these in participants with and without hyperglycaemia and investigated their relationship with two polymorphisms (rs17300539 and rs266729) reported to affect adiponectin values. In the fourth, we determined E-selectin levels in 307 participants, compared these in participants with and without hyperglycaemia and assessed their effect on CIMT. Results The recommended HbA1c cut-off was not sensitive enough to detect DM. Using FBG, 117 (14%) participants were diagnosed with DM and 50% had an HbA1c of  6.5%; using OGTT 147 (18%) had DM and 46% had an HbA1c of  6.5%. Comparing HbA1c to FBG and OGTT, a cut-off of 6.1% gave a better sensitivity and specificity (area under curve (AUC) 0.85 and 0.82 respectively). Also, the recommended HbA1c cut-off to detect prediabetes was not appropriate and we determined that 5.75% was best. However, the low sensitivity and specificity (64.8% and 60.4% respectively for the derivation and first validation sample and 59.6% and 69.8% for the second validation sample), confirmed that HbA1c alone would miss a significant number of prediabetics. Hmw-adiponectin levels were not affected by glycaemia (median 11.6 g/mL in normoglycaemia vs. 10.5 g/mL in hyperglycaemia; p=0.3060) nor by two common polymorphisms. Using robust correlations, a significant correlation was found between hmw-adiponectin and high density lipoprotein cholesterol (HDL-c) (r=0.45; 95%CI: 0.27-0.59), which was similar in both normo-and hyperglycaemia (p>0.99). This association was attenuated in robust linear regressions adjusted for gender and adiposity. Eselectin levels were significantly higher in hyperglycaemia (median 139.8 g/L vs. 118.8 g/L in normoglycaemia; p=0.0007) but not associated with CIMT. Significant correlations were found between E-selectin and age, markers of glycaemia and inflammation, central obesity and lipid variables. Associations remained significant only with age, hyperglycaemia and C-reactive protein (CRP) in multivariable robust linear regression models. In similar regressions models, age and gender were the main predictors of CIMT, which was not associated with E-selectin. Conclusion The international HbA1c cut-offs recommended to detect DM and prediabetes were not appropriate in our population. Though a cut-off of 6.5% to diagnose DM is a good diagnostic tool with high specificity, the low sensitivity limits its screening use. Similarly, recommended HbA1c values to detect prediabetes may underestimate the true numbers. This emphasizes the importance of local evidence-based values being established. Additionally, hmw-adiponectin was not affected by glycaemia or polymorphisms, but correlated significantly with HDL-c which may explain its beneficial cardiovascular effect. Though Eselectin was influenced by glycaemia, possibly reflecting early endothelial damage, it did not correlate with CIMT, which was determined by age and male gender.
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    Interactions between the noradrenergic and dopaminergic neurotransmitter systems in the rat brain
    (Stellenbosch : Stellenbosch University, 1990) Allin, Rosemary; Taljaard, J. J. F.; Russel, V. A.; Stellenbosch University. Faculty of Medicine and Health Sciences. Department of Pathology.
    ENGLISH ABSTRACT: The development of a dissection technique enabled the nucleus accumbens to be dissected into six and the striatum into eighteen discrete areas. The concentration of monoamines in these areas was analysed by high performance liquid chromatography (HPLCl with electrochemical detection. The distribution of the different monoamines in the nucleus accumbens was not identical. In general, concentrations were found to be low in the rostral area of the nucleus accumbens. Marked differences were observed in the medial area. Dopamine (DA) levels were significantly lower in the ventrorostral than in the dorsorostral nucleus accumbens and high in both medial and caudal areas. Noradrenaline (NA) and serotonin (5-HT) levels were considerably lower than those of DA. The NA concentration was highest in the caudal area of the nucleus accumbens and the 5-HT concentration was highest in the ventrocaudal area There was evidence for a rostrocaudal decrease in DA and 5-UT turnover in the nucleus accumbens. In the striatum, DA levels were higher rostrally than caudally, the lowest levels being found in the globus pallidus. NA levels were low throughout the striatum but significantly higher in the globus pallidus 5-HT and 5-hydroxyindole acetic acid (5-HIAA) levels were higher ventrally than dorsally and ancreased along the rostrocaudal axis. Selective lesioning of the locus coeruleus (LC) noradrenergic neurons by the administration of N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4) or by direct infusion of 6-hydroxydopamine (6-OHDA) resulted in a decrease in NA concentration in the rostral area or the nucleus accumbens. DA and 5-HT levels were not affected by those lesions. DSP4 lesions caused increased 3,4-dihydroxyphenyl acetic (DOPAC) turnover in the ventromedial and ventrocaudal areas, indicating increased catechol-O-methyitransferase (COMT) activity in these areas, 6-OHDA lesions of the medial forebrain bundle (MFB) resulted in decreased NA and DA levels in all areas of the nucleus accumbens. DA turnover was increased, indicating increased monoamine oxidase (MAO) activity in the medial and caudal areas after MFB lesions. lncreased 5-HIAA/5-HT ratios were also found in the medial and caudal areas. The distribution or DA DI and D2 receptors in the nucleus accumbens was determined by means of radioligand binding assays [3H|SCH23390 was used to label DA D1 receptors and [3H]spiperone was used for DA D2 receptors. The distribution of DA D1 and D2 receptors was not superimposable although there was considerable overlap. DA D1 receptor density roughly followed the DA innervation, being low rostrally and high medially and caudally. There were no dorsoventral differences. In the ventrorostral area it appears that relatively few, more active neurons can activate a similar number or postsynaptic DA D1 receptors. DA D2 receptor density was lowest in the ventrorostral area, highest in the dorsomedial area and similar in the remaining areas of the nucleus accumbens. Chronic treatment with desipramine resulted in no significant changes in DA D1 or D2 receptor number or affinity in the nucleus accumbens, therefore increased dopaminergic transmission occurring after chronic antidepressant treatment would appear not to be due to direct changes in DA receptor binding.
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    Investigation of the role of vitamin D metabolism in South African breast cancer patients using a pathology-supported genetic testing platform
    (Stellenbosch : Stellenbosch University., 2020-03) Okunola, Abisola Oyedele; Kotze, Maritha J.; Erasmus, Rajiv T.; Zemlin, Annalise E.; Torrorey-Sawe, Rispah; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology. Chemical Pathology.
    ENGLISH ABSTRACT: The high global breast cancer incidence drives the development of novel genomic approaches for disease prevention and targeted treatment. Towards this goal, a pathology-supported genetic testing (PSGT) platform was established to facilitate risk management of non-communicable diseases across the continuum of care, ranging from early-stage to metastatic disease and cancer survivorship. The causes and consequences of low vitamin D levels recently reported in the majority of postmenopausal breast cancer patients treated with aromatase inhibitors at the Tygerberg Academic Hospital, in the Western Cape Province of South Africa, were addressed in this study using PSGT to translate genomic findings into clinical practice. The aim was to determine the relationship between clinical characteristics, tumour histopathology and genetic variation underlying vitamin D metabolism in postmenopausal breast cancer patients at increased risk of osteoporosis, identified as a significant co-morbidity in the study population. Clinical and lifestyle information of 116 postmenopausal women with known vitamin D status diagnosed with breast carcinoma between 2014 and 2017 was extracted from a central genomics database linked to a biobank of DNA samples extracted from blood. Whole exome sequencing (WES) was performed on the Ion Torrent platform, followed by variant calling of vitamin D-related genes, while simultaneously assessing BRCA1/2 mutation status. Allele-specific real-time polymerase chain reaction (PCR), Sanger sequencing and long-range nanopore sequencing using the pocket-size MinION device were used to verify the WES results and to screen for variants in the vitamin D receptor (VDR) and E-cadherin (CDH1) genes beyond the coding regions covered by WES. Seasonal variation (p = 0.009) and high body mass index (BMI) (p = 0.032) contributed significantly to vitamin D levels, with the lowest values recorded during winter. WES initially performed in 10 breast cancer patients selected based on vitamin D levels at extreme upper and lower ranges, identified GC rs4588 (c.1364C>A, T455K) as a potential contributing factor vitamin D deficiency in the five patients with ultra-low vitamin D levels (≤12 ng/mL). However, 2/5 patients with levels in the upper extreme of vitamin D (>30 ng/mL), also tested positive for this variant and no significant association was detected after extended genotyping in 100 South African patients using real-time PCR. Sanger sequencing subsequently performed in 14 breast cancer patients diagnosed with osteoporosis prior to initiation of aromatase inhibitor therapy, highlighted the potential significance of genetic variation in the VDR gene. WES analysis of VDR in an extended sample of 55 breast cancer patients furthermore confirmed the significant effect of genetic variation in this gene on bone health (p < 0.001). The CDH1 gene known to be activated by VDR was furthermore analysed in patients stratified by tumour type. CDH1 c.G671A (p.R224H) detected in a breast cancer patient with invasive carcinoma of no special type (ICNST) was classified as benign, since pathogenic germline CDH1 variants are associated with invasive lobular carcinoma and diffuse gastric cancer, but not ICNST. CDH1 c.A1298G (p.D433G) was detected in a patient with invasive lobular carcinoma together with a pathogenic BRCA1 variant detected by WES. Although this finding supports a likely benign classification for CDH1 p.D433G as reported in the international ClinVar database, a family history of stomach cancer raised the possibility of a CDH1 modifier gene effect on BRCA1 gene expression. New insights gained through integration of pathology and genomic findings were incorporated into a pharmaco-diagnostic algorithm applicable to hormone receptor-positive postmenopausal breast cancer patients. The PSGT platform facilitated interpretation of research results of study participants through use of WES and recommendation of genetic counselling where appropriate.
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    Methodological issues around the validation of models for predicting diabetes risk in developing countries
    (Stellenbosch : Stellenbosh University, 2016-12) Masconi, Katya Laura; Kengne, Andre Pascal; Erasmus, Rajiv T.; Matsha, Tandi E.; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Chemical Pathology.
    ENGLISH SUMMARY: Background: Multivariable diabetes risk prediction models have the potential to contribute to screening strategies, combining several risk factors to predict undiagnosed diabetes or future risk of developing diabetes. The focus of this study is the prediction of undiagnosed diabetes and diabetes risk prediction in a developing country where no population-specific diabetes risk prediction model currently exists. Existent models have been developed in unrelated populations with different disease prevalence, predictor weightings and methods used for risk factor determination and diabetes diagnosis. For accurate diabetes risk prediction in the mixed ancestry population of Bellville South, Cape Town, methodological issues regarding the validation and performance of these models needs to be addressed. Methodology: Cross-sectional data from the Cape Town Bellville South cohort was used for this study. Missing data in risk prediction research was investigated through a systematic review and a number of imputation methods were explored to deal with missing data in this dataset. Models were identified via recent systematic reviews and validated in the mixed-ancestry population. Discrimination was assessed and compared using the C-statistic and calibration was assessed via calibration plots. Model recalibration in diabetes risk prediction was investigated through a systematic review. In an effort to improve model performance in the new setting, model recalibration and updating strategies were used and performance was compared before and after implementation. Results: The study sample consisted of 1256 individuals, of whom 173 were excluded due to previously diagnosed diabetes. Of the final 1083 individuals, 329 (30.4%) had missing data. Deletion resulted in the lowest model performance and simple imputation, the simplest method, resulted in the highest model performance and was employed for further analysis. A systematic review highlighted the gross underreporting and mishandling of missing data in diabetes risk prediction research. Original model performance during validation was poor-to-average, with both over- and underestimation present: Cambridge [C-statistic: 0.67 (0.62-0.72); E/O: 1.81 (1.09-2.52)], Kuwaiti [C-statistic: 0.68 (0.63-0.73); E/O: 0.72 (0.43-1.12)], Omani [C-statistic: 0.66 (0.61-0.70); E/O: 1.28 (0.63-1.93)], Rotterdam [C-statistic: 0.64 (0.59-0.69); E/O: 0.54 (0.50-1.04)] and Simplified Finnish [C-statistic: 0.67 (0.62-0.71); E/O: 0.26 (0.13-0.39)] diabetes risk prediction models. Recalibration, as shown through a systematic review, was undertaken only in models predicting incident diabetes, and was reported in 22.9% of validation studies, with 77.8% achieving an increase in model performance. Updating results applied to this validation dataset showed an increase in both discrimination and calibration in varying levels across all five models. Overall, the re-estimation of the Cambridge diabetes risk model yielded the best model performance [C-statistic: 0.71 (0.67 – 0.75); E/O: 1.00 (0.86 – 1.17)]. Discussion and conclusion: The frequency of missing data, underreporting and mishandling of missing data, complexity of updating methods and overall model performance of validated models in new settings highlight the challenges in diabetes risk prediction research. This is the first validation study of prevalent diabetes risk prediction models in Sub-Saharan Africa and highlighted important methodological issues. While both simpler imputation and updating methods resulted in similar predictive utility when compared to more complex techniques, model performance was not increased sufficiently to suggest recommendation.
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    Molecular investigation of genetic and environmental factors contributing to obesity in adolescent learners residing in the semi-urban/rural areas of the Western Cape Province, South Africa
    (Stellenbosch : Stellenbosch University, 2012-12) Yako, Yandiswa Yolanda; Erasmus, Rajiv T.; Matsha, Tandi; Janse van Rensburg, Susan; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology. Chemical Pathology.
    ENGLISH ABSTRACT: Background/Aims: Obesity has increased rapidly in South African children and adolescents with significant variability observed among racial groups. Genes that regulate appetite have been studied in different populations worldwide, but their role in obesity among South African adolescents is unknown. The present study aimed at investigating the role of these genes, and their combined effect with physical activity in the development of obesity among South African adolescents. Methods: A total of 1564 South African school learners of Caucasian (n= 146), Mixed Ancestry (n= 872) and Black African (n= 537) ethnic groups were recruited for a research project that aimed to elucidate diabetes and the metabolic syndrome in children and adolescents attending schools in periurban areas of the Western Cape. The present case-control study included 227 obese-overweight (115 Black Africans and 112 Mixed Ancestry), and 204 normal weight (94 Black Africans and 110 Mixed Ancestry) adolescents learners. The learners were genotyped for nine polymorphisms (LEP: 19G>A, Lys36Arg, Val94Met; LEPR: Lys109Arg; Gln223Arg, Lys656Asn; CART: c.160-33G>A, c.499delA, and c.517A>G; GHRL: Leu72Met; and MC3R: Thr6Lys, Val81Ile) using allele-specific restriction enzyme analysis and automated sequencing. Genotype and haplotype associations with anthropometric variables such as body mass index (BMI), waist, hip, and mid-upper-arm circumferences (WC, HC, MUAC), and metabolic traits (fasting blood glucose, high density lipoproteincholesterol, total cholesterol), and blood pressure were further conducted. Furthermore, the type and frequency of physical activity was assessed by means of structured questionnaires; and its effect on obesity-related variables investigated in learners that were genotyped for the MC3R Thr6Lys and Val81Ile polymorphisms. Results: In a stepwise backward logistic regression analysis (containing age, gender, and LEP, LEPR, CART and GHRL polymorphisms), CART c.517A>G was independently significantly associated with obesity (OR= 5.98; 95%CI= 2.02, 21.27). CART c.517G carriers had higher MUAC (b coefficient= 1.88; 95%CI= 0.31, 3.44) while the LEPR 109Arg allele was significantly associated with decreased BMI (b coefficient = -2.36; 95%CI= -4.24, -0.47), WC (b coefficient = -5.66; 95%CI= -9.89, -1.44) and MUAC (b coefficient = -1.61; 95%CI= -3.00, -0.22); after adjusting for age, gender, and ethnicity. The haplotype containing the three LEP polymorphisms (A-A-A compared to the reference G-A-G haplotype) increased BMI (p= 0.0155), MUAC (p= 0.0146), and HC (p= 0.0128). The minor alleles of the MC3R polymorphisms decreased BMI, HC, WC, MUAC and TC; whilst only the Thr6Lys was associated with systolic and diastolic blood pressure (p= 0.0047 and 0.0027, respectively) in Mixed Ancestry learners. Doing house chores was associated with lower total cholesterol, independently and in the presence of the 81Ile allele (b coefficient = -0.355; 95%CI= 0.148, 0.561). Conclusion: To our knowledge, this is the first study that reports CART c.517A>G polymorphism as a risk factor for obesity in adolescents. Furthermore, the present study demonstrated that the MC3R polymorphisms had a positive effect on total cholesterol, which was further enhanced in physically active individuals. Similar to other studies, LEPR Lys109Arg and LEP polymorphisms were associated with variations in obesity-related variables among Black African and Mixed Ancestry South African learners.
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    Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population
    (Stellenbosch : Stellenbosh University, 2015-12) Vergotine, Zelda; Erasmus, Rajiv T.; Matsha, Tandi E.; Pillay, Tahir S.; Kotze, Maritha J.; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Chemical Pathology.
    ENGLISH ABSTRACT: Background: The aetiopathogenesis of type 2 diabetes and the associated insulin resistance have been shown to have a strong genetic basis. Several genetic variants of the peroxisome proliferatoractivated receptor gamma (PPARG) and the insulin receptor substrate (IRS) 1 genes have been associated with the metabolic states of obesity, insulin resistance and type 2 diabetes in Caucasian populations. Furthermore, insulin resistance is strongly associated with diabetes and subsequent cardiovascular disease. These are increasingly common in low- to middle -income countries, including South Africa. Limited information is currently available regarding genetic associations with insulin resistance in African populations. Objectives: (1) To identify subjects with insulin resistance and determine the frequencies of the single nucleotide polymorphisms in the PPARG and IRS1 genes and examine the associated risk of insulin resistance and type 2 diabetes mellitus in a mixed-ancestry South African population. (2) To investigate the relationship between indices of insulin resistance and carotid intima media thickness, a marker of subclinical cardiovascular disease/atherosclerosis. Methods: A total of 856 (235 males) mixed-ancestry adults drawn from an urban community of Bellville South, Cape Town were genotyped for PPARG Pro12Ala (rs1801282, G>C), Pro115Gln (rs1800571, G>T), Val290Met (rs72551362, G>A), Pheu388Leu (rs72551363, T>A), Arg397Cys (rs72551364, C>T), His449His (rs3856806, C>T) and IRS1 Gly972Arg (rs 1801278, G>A). The oral glucose tolerance test was performed and cardiometabolic risk factors measured. Insulin resistance was estimated by the homeostasis model assessment of insulin resistance, the homeostasis model assessment of functional beta-cells, the quantitative insulin-sensitivity check index, the fasting insulin resistance index and the glucose/insulin ratio. Carotid intima media thickness was measured in longitudinal section at the far wall of the distal common carotid arteries, 2 cm from the bifurcation, at three consecutive end-points, 5-10 mm apart. Results: The genotype frequencies of PPARG Pro12Ala, IRS1 Gly972Arg and PPARG His449His were 10,4%, 7,7% and 23,8% respectively. No mutations were found for PPARG Pro115Gln, Val290Met, Pheu388Leu and Arg379Cys. In a model containing both PPARG Pro12Ala and IRS1 Gly972Arg alleles and their interaction term, the presence of the PPARG Pro12 resulted in a 64% risk of prevalent type 2 diabetes mellitus and was associated with higher 2 hour post-OGTT insulin levels in subjects with normoglycaemia. The PPARG Pro12 was associated with insulin resistance and interacted with IRS1 Gly972Arg, increasing the risk of type 2 diabetes mellitus. The PPARG His449His allele T frequency was about 14% and in an additive genetic model significantly reduced the risk of diabetes by 44%. After adjustment for age, gender, body mass index and diabetes status, the fasting plasma glucose (β=0,087;p=0,042) and glucose/insulin ratio (β=0,026; p=0,026) were associated with carotid intima media thickness. However, the effect on the overall model performance was marginal, R2<29,7%. Conclusion: The PPARG Pro12 was associated with insulin resistance and showed a gene-gene interaction with the unfavorable polymorphism IRS1 Gly972Arg, leading to an increased risk of type 2 diabetes mellitus. In contrast, the PPARG His449His T allele showed a protective effect against the risk of developing diabetes. Furthermore, indices of insulin resistance such as homeostatis model assessment of insulin resistance, quantitative insulin-sensitivity check index, fasting insulin resistance index and the glucose/insulin ratio were weakly associated with carotid intima media thickness in the risk stratification of cardiovascular disease in this population.
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    Mutational analysis of the solute carrier family 11 member 1 gene (SLC11A1) implicated in iron transport
    (Stellenbosch : Stellenbosch University, 2003-12) Zaahl, Monique G. (Monique Glenda); Kotze, Maritha J.; Warnich, L.; Winter, T. A.; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences.
    ENGLISH ABSTRACT: The solute carrier family 11 member 1 gene (SLC11A 1) is a divalent metal ion transporter with various pleiotropic effects on macrophage function. This gene that regulates iron, and is also regulated by cellular iron levels, has previously been linked to many infectious and autoimmune diseases. In this analysis, in vitro studies using the luciferase reporter system as well as case-control association studies were applied to investigate the significance of SLC11 A1 allelic variation in patients with diverse disease phenotypes. For in vitro studies, five different SLC11A 1 promoter constructs were generated, followed by transfection into U937 and THP-1 cells. The inserted fragments included two previously described alleles (alleles 2 and 3), two novel alleles identified in this study (alleles 8 and 9) and a C to T point mutation at nucleotide position -237 in the presence of allele 3. The most striking finding was the opposite effect observed for allele 3 in the presence of the -237C~ T polymorphism, similar to that of allele 2. Although the SLC11A 1 gene has previously been implicated in iron transport, we have demonstrated, for the first time, that the various alleles investigated cause differential expression of the gene upon iron loading. Association studies were performed by investigating diseases including oesophageal cancer (DC), inflammatory bowel disease (lBO) and hereditary haemochromatosis (HH) (or primary iron overload). Significant associations (P
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    Reference intervals for common chemistry and haematology laboratory tests in a healthy Kenyan population: variation with age, sex, BMI and comparison with a South African
    (2020-12) Omuse, Geoffrey Amuka; Erasmus, Rajiv; Ichihara, Kiyoshi; Premji, Zulfiqarali; Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology. Chemical Pathology.
    ENGLISH ABSTRACT: Introduction Laboratory results play an important role in assessment of one’s health status including detection of sub-clinical disease. Reference intervals (RIs) have been shown to vary across different populations due to various reasons including ethnic and racial differences, differences in reference populations and statistical approaches used in deriving the RIs. The International Federation of Clinical Chemistry committee on reference intervals and decision limits has been carrying out a global study aimed at harmonizing RIs. Kenya is the only country in East Africa that participated in this study and therefore the derived RIs will serve as a reference point for laboratories in the region. It is also important to identify what factors cause variations in RIs and whether partitioning based on age or sex will make them more specific. We subsequently compared our RIs with those from South Africa also derived as part of the global RI study. Methods Recruitment of study participants in Kenya was carried out between January and October 2015 in several counties after obtaining informed consent. Inclusion of participants was limited to healthy adults aged 18-65 years stratified into 4 age groups: 18-29, 30-39, 40-49 and 50-65 years. Haematology tests were performed at the PathCare laboratories in Nairobi, Kenya while all other analysis was done at the PathCare reference laboratory in Cape Town, South Africa. For purposes of the global RI study, all participating laboratories received a panel of sera that had assigned values to enable recalibration of reference values (RVs) and alignment across different countries. RIs were determined using both parametric and non-parametric methods before and after applying the latent abnormal values exclusion (LAVE) method. Results Out of 596 volunteers, 533 met the inclusion criteria: 260 (48.8%) males and 273 (51.2%) females. The prevalence of metabolic syndrome (MetS) was 25.6% and less than 1% of participants had reduced estimated glomerular filtration rate (eGFR). Sex-specific RIs were required for uric acid, creatinine, total bilirubin (TBil), total cholesterol (TC), transaminases, transferrin, transferrin saturation and immunoglobulin-M. Age-specific RIs were required for glucose and triglyceride for both sexes, and for urea, magnesium (Mg), TC, HDL-cholesterol ratio, alkaline phosphatase (ALP), and ferritin for females. Kenyan RIs were comparable to those of other countries participating in the global study with a few exceptions such as higher ULs for TBil and c-reactive protein (CRP). South African RIs for uric acid, TC, low density lipoptotein cholesterol, alanine transaminase, lactate dehydrogenase, ALP, albumin, Mg, thyroid stimulating hormone and prostate specific antigen were lower than the Kenyan RIs. Conclusion Kenyan RIs for several analytes were established using a harmonized protocol from well-defined reference individuals. Given the rigour with which the study was conducted, the derived RIs will provide a useful reference for laboratories in sub-Saharan Africa that are looking for RIs for common haematology and biochemistry tests. For most analytes, harmonization of RIs between Kenyans and South Africans of African ancestry was not possible as they could result in misclassification of individuals as either diseased or healthy given the differences seen.