Phylogenetic analysis of Alternaria spp. associated with apple core rot and citrus black rot in South Africa

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dc.contributor.authorKang J.-C.
dc.contributor.authorCrous P.W.
dc.contributor.authorMchau G.R.A.
dc.contributor.authorSerdani M.
dc.contributor.authorSong S.-M.
dc.date.accessioned2011-05-15T15:57:24Z
dc.date.available2011-05-15T15:57:24Z
dc.date.issued2002
dc.description.abstractDry core rot of apple (DCR) and Alternaria black rot of citrus (ABR) have in the past respectively been ascribed to Alternaria alternata and A. citri. In recent years, however, it has been speculated that several other species of Alternaria could also be associated with these diseases. In an attempt to elucidate the identity of these taxa, 25 isolates associated with DCR, and 26 isolates associated with ABR were selected for molecular characterisation. Nucleotide sequences of 1116 sites including the histone gene section and the internal transcribed spacers (ITS 1 and 2) of the rRNA gene were determined for these isolates. The gene trees generated from the individual and combined data sets using maximum parsimony, maximum likelihood and neighbour-joining analysis methods distinguished five clades with strong bootstrap support, namely Alternaria sp., A. arborescens, A. infectoria, A. tenuissima, and a clade containing isolates variable in morphology, referred to as the Alternaria group. In the alignment of the combined ITS and histone data set, unique transition/transversion substitutions, as well as positional insertions and deletions were observed for each of the above clades. In addition, key sequences in the form of serial composing nucleotides in both the ITS and histone sections of the alignment were also discovered for the molecular identification of A. arborescens, A. infectoria and A. tenuissima. The final phylogeny also indicated that no host specificity existed among the species associated with these two post-harvest disease complexes. Contrary to the host specificity observed on leaf diseases of these hosts in the field, it appears that the post-harvest diseases are the result of adverse storage conditions and opportunism of different small-spored Alternaria spp.
dc.description.versionArticle
dc.identifier.citationMycological Research
dc.identifier.citation106
dc.identifier.citation10
dc.identifier.issn9537562
dc.identifier.other10.1017/S0953756202006524
dc.identifier.urihttp://hdl.handle.net/10019.1/10384
dc.subjectfungal disease
dc.subjecthost specificity
dc.subjectphylogenetics
dc.subjectSouth Africa
dc.subjectAlternaria
dc.subjectAlternaria alternata
dc.subjectAlternaria arborescens
dc.subjectAlternaria citri
dc.subjectAlternaria tenuissima
dc.subjectCitrus
dc.subjectFungi
dc.subjectLewia infectoria
dc.subjectMalus x domestica
dc.titlePhylogenetic analysis of Alternaria spp. associated with apple core rot and citrus black rot in South Africa
dc.typeArticle
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