Characterization of HIV type 1 genetic diversity among South African participants enrolled in the AIDS Vaccine Integrated Project (AVIP) Study

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dc.contributor.authorPapathanasopoulos M.A.
dc.contributor.authorVardas E.
dc.contributor.authorWallis C.
dc.contributor.authorGlashoff R.
dc.contributor.authorButto S.
dc.contributor.authorPoli G.
dc.contributor.authorMalnati M.
dc.contributor.authorClerici M.
dc.contributor.authorEnsoli B.
dc.date.accessioned2011-05-15T16:03:20Z
dc.date.available2011-05-15T16:03:20Z
dc.date.issued2010
dc.description.abstractThe genetic diversity of HIV-1 strains circulating among HIV-1-infected South Africans was investigated in a cohort of 420 individuals enrolled as part of the AIDS Vaccine Integrated Project (AVIP) study. Representative samples (10%) were randomly selected from treatment-naive participants. Viral RNA was extracted for reverse transcriptase-initiated amplification and population-based sequencing of partial pol (encompassing protease and reverse transcriptase) and full-length integrase. Overall, HIV-1 sequences confirmed that 97.1% and 96.9% were HIV-1 subtype C in pol and integrase, respectively. Two participants were infected with unique A1/C and C/A1 recombinants in pol/integrase. Further pol sequence analysis identified mutation patterns associated with high level resistance to NNRTIs in two participants, whereas no primary mutations conferring resistance to integrase inhibitors were detected. The predominance of HIV-1 subtype C in South African populations is therefore confirmed in the AVIP cohort finalized for testing preventive or therapeutic vaccines against HIV-1 infection. © 2010, Mary Ann Liebert, Inc.
dc.description.versionArticle
dc.identifier.citationAIDS Research and Human Retroviruses
dc.identifier.citation26
dc.identifier.citation6
dc.identifier.issn8892229
dc.identifier.other10.1089/aid.2009.0281
dc.identifier.urihttp://hdl.handle.net/10019.1/12584
dc.subjectetravirine
dc.subjectintegrase
dc.subjectintegrase inhibitor
dc.subjectnonnucleoside reverse transcriptase inhibitor
dc.subjectPol protein
dc.subjectproteinase
dc.subjectvirus RNA
dc.subjectadult
dc.subjectarticle
dc.subjectCD4 lymphocyte count
dc.subjectfemale
dc.subjectgene mutation
dc.subjectgene sequence
dc.subjectgenetic variability
dc.subjecthuman
dc.subjectHuman immunodeficiency virus 1
dc.subjectHuman immunodeficiency virus 1 infection
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectNegro
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectphylogenetic tree
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectSouth Africa
dc.subjectAdult
dc.subjectCluster Analysis
dc.subjectFemale
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectHIV Infections
dc.subjectHIV-1
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMolecular Sequence Data
dc.subjectpol Gene Products, Human Immunodeficiency Virus
dc.subjectRNA, Viral
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology
dc.subjectSouth Africa
dc.titleCharacterization of HIV type 1 genetic diversity among South African participants enrolled in the AIDS Vaccine Integrated Project (AVIP) Study
dc.typeArticle
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