Evaluation of polygalacturonase activity in Saccharomyces cerevisiae wine strains

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dc.contributor.authorFernandez-Gonzalez M.
dc.contributor.authorUbeda J.F.
dc.contributor.authorVasudevan T.G.
dc.contributor.authorOtero R.R.C.
dc.contributor.authorBriones A.I.
dc.date.accessioned2011-05-15T16:04:56Z
dc.date.available2011-05-15T16:04:56Z
dc.date.issued2004
dc.description.abstractA total of 61 S. cerevisiae strains, 60 of them isolated from wine ecosystems, were evaluated for the presence of the gene encoding endopolygalacturonase (PGU1) and for polygalacturonase (PG) activity. Nine strains lack the gene PGU1 and did not exhibit PG activity on plate assays. Of the 52 strains showing an amplified band corresponding to the size of PGU1 gene, only 36 degraded polygalacturonic acid (PGA) and 17 did not degrade it at any of the pH values used. The coding region of the PGU1 gene (ORF YJR153w) was not present in some PG activity negative strains. The S. cerevisiae UCLMS-39 strain was selected for its specific activity at different pHs, temperatures and oenological parameters. The temperature and pH optima were 50°C and 3.5-5.5 respectively and it was only affected by ethanol. The PGU1 gene was cloned and sequenced. The production of a biologically functional endoPG in S. cerevisiae UCLMS-39 brings us a step closer to improving the qualities of outstanding enological yeasts naturally lacking PG activity. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationFEMS Microbiology Letters
dc.identifier.citation237
dc.identifier.citation2
dc.identifier.issn3781097
dc.identifier.other10.1016/j.femsle.2004.06.042
dc.identifier.urihttp://hdl.handle.net/10019.1/12897
dc.subjectalcohol
dc.subjectpolygalacturonase
dc.subjectpolygalacturonic acid
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdegradation
dc.subjectDNA sequence
dc.subjectenzyme activity
dc.subjectfungal strain
dc.subjectgene expression
dc.subjectgene overexpression
dc.subjectgene sequence
dc.subjectgenetic code
dc.subjecthaploidy
dc.subjectmolecular cloning
dc.subjectmolecular size
dc.subjectnonhuman
dc.subjectpH
dc.subjectpriority journal
dc.subjectSaccharomyces cerevisiae
dc.subjecttemperature sensitivity
dc.subjectviniculture
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectCloning, Molecular
dc.subjectPectins
dc.subjectPolygalacturonase
dc.subjectPolymerase Chain Reaction
dc.subjectSaccharomyces cerevisiae
dc.subjectWine
dc.subjectSaccharomyces
dc.subjectSaccharomyces cerevisiae
dc.titleEvaluation of polygalacturonase activity in Saccharomyces cerevisiae wine strains
dc.typeArticle
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