The influence of exposure history to lead on the lysosomal response in Eisenia fetida (Oligochaeta)

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dc.contributor.authorReinecke A.J.
dc.contributor.authorReinecke S.A.
dc.date.accessioned2011-05-15T16:01:36Z
dc.date.available2011-05-15T16:01:36Z
dc.date.issued2003
dc.description.abstractIn this study groups of the epigeic earthworm species Eisenia fetida were exposed experimentally for different periods to sublethal concentrations of Pb(NO3)2. The lysosomal membrane fragility response of coelomocytes was used as a biomarker to explore the cytotoxity of Pb by determining the neutral red retention times (NRRTs) and correlating them with different body loads of Pb. Previously unexposed worms exposed to different low concentrations of Pb(NO3)2 for 4 weeks demonstrated a clear dose-response relationship between NRRTs and body burdens of Pb. The NRRTs of growing worms with a previous (ancestral) exposure history, exposed to an elevated concentration of Pb and measured over time, fluctuated around a mean of 41min and no dose-related relationship could be demonstrated. Worms with a long-term history of exposure to Pb of more than 36 months had a mean body burden of 177.5±61.5mg/kg Pb and a mean NRRT of 57.8±3.1min, which was significantly lower than that of uncontaminated, control worms with a mean time of 61.9±3.4min and a mean body burden of 86.3±47.2mg/kg. No significant differences were found between weight changes of "clean" and preexposed worms when they were experimentally exposed to sublethal concentrations of Pb. This study provided evidence that the lysosomal biomarker response for Pb may not persist at the same level in worms with a previous history of Pb exposure, possibly making it less sensitive as a tool for comparing responses to Pb in field populations of earthworms. © 2003 Elsevier Science (USA). All rights reserved.
dc.description.versionArticle
dc.identifier.citationEcotoxicology and Environmental Safety
dc.identifier.citation55
dc.identifier.citation1
dc.identifier.issn1476513
dc.identifier.other10.1016/S0147-6513(02)00086-6
dc.identifier.urihttp://hdl.handle.net/10019.1/12062
dc.subjectbiological marker
dc.subjectlead
dc.subjectearthworm
dc.subjectecotoxicology
dc.subjectlead
dc.subjectphysiological response
dc.subjectpollution exposure
dc.subjectsoil pollution
dc.subjectannelid worm
dc.subjectarticle
dc.subjectbody burden
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectcorrelation function
dc.subjectcytotoxicity
dc.subjectearthworm
dc.subjectenvironmental exposure
dc.subjectlysosome membrane
dc.subjectnonhuman
dc.subjectworm
dc.subjectAnimals
dc.subjectBody Burden
dc.subjectEnvironmental Exposure
dc.subjectLead
dc.subjectLysosomes
dc.subjectOligochaeta
dc.subjectSoil Pollutants
dc.subjectEisenia fetida
dc.subjectPheretima sieboldi
dc.titleThe influence of exposure history to lead on the lysosomal response in Eisenia fetida (Oligochaeta)
dc.typeArticle
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