Quantification of glutathione, catechin and caffeic acid in grape juice and wine by a novel ultra-performance liquid chromatography method

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dc.contributor.authorFracassetti, D.en_ZA
dc.contributor.authorLawrence, N.en_ZA
dc.contributor.authorTredoux, A. G. J.en_ZA
dc.contributor.authorTirelli, A.en_ZA
dc.contributor.authorNieuwoudt, Heleneen_ZA
dc.contributor.authorDu Toit, W. J.en_ZA
dc.date.accessioned2011-10-13T16:59:19Z
dc.date.available2011-10-13T16:59:19Z
dc.date.issued2011-05
dc.description.abstractThis research aimed at the development and validation of an ultra-performance liquid chromatography (UPLC) method for the quantification of glutathione (GSH) in grape juice and in white wine after derivatisation with para-benzoquinone. The phenolic compounds catechin and caffeic acid that occur in white wine and have antioxidant effects, are also quantified in the same analysis. Catechin is the basic monomeric unit of grape and wine tannins and caffeic acid, when esterified with tartaric acid, plays a relevant role in Grape Reaction Product (GRP) formation. The analytical method proposed showed good linearity, repeatability and intermediate repeatability, as well as high recovery (>85%). It was applied for the quantification of GSH, catechin and caffeic acid in South African juices (12) and white wines (43). This novel method will have a large impact on the time and costs of the analyses for the wine industry through enabling rapid routine quantification of GSH, catechin and caffeic acid. © 2011 Elsevier Ltd. All rights reserved.en_ZA
dc.description.versionArticle
dc.identifier.citationFood Chemistry
dc.identifier.citation128
dc.identifier.citation4
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79958041263&partnerID=40&md5=18824440f614f0d70e9b5d352091429d
dc.identifier.issn3088146
dc.identifier.other10.1016/j.foodchem.2011.04.001
dc.identifier.urihttp://hdl.handle.net/10019.1/17064
dc.titleQuantification of glutathione, catechin and caffeic acid in grape juice and wine by a novel ultra-performance liquid chromatography method
dc.typeArticle
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