PGU1 gene natural deletion is responsible for the absence of endo-polygalacturonase activity in some wine strains of Saccharomyces cerevisiae

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dc.contributor.authorDivol, Benoiten_ZA
dc.contributor.authorVan Rensburg, P.en_ZA
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2007
dc.description.abstractThe PGU1 gene encodes an endo-polygalacturonase enzyme in Saccharomyces cerevisiae. The literature reports that most S. cerevisiae strains possess this gene, despite a wide range of enzyme activity levels. Nevertheless, a few wine strains lack the PGU1 gene. We investigated the PGU1 locus sequence in these strains. The results indicated that the gene had been replaced by a partial Ty mobile element, whereas the gene promoter was still at the expected location. As all the strains lacking the PGU1 gene experienced the same phenomenon, it was tempting to hypothesize a common phylogenetic origin. However, fingerprints only allowed grouping of a few of them within one cluster. © 2007 Federation of European Microbiological Societies.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation7
dc.identifier.citation8
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2007.00284.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12622
dc.subjectpolygalacturonase
dc.subjectarticle
dc.subjectenzyme activity
dc.subjectgene
dc.subjectgene cluster
dc.subjectgene deletion
dc.subjectgene location
dc.subjectgene locus
dc.subjectgene sequence
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectPGU1 gene
dc.subjectphylogeny
dc.subjectpromoter region
dc.subjectSaccharomyces cerevisiae
dc.subjectwine
dc.subjectCluster Analysis
dc.subjectDNA Fingerprinting
dc.subjectDNA, Fungal
dc.subjectInterspersed Repetitive Sequences
dc.subjectPhylogeny
dc.subjectPolygalacturonase
dc.subjectPromoter Regions (Genetics)
dc.subjectRecombination, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Analysis, DNA
dc.subjectWine
dc.subjectSaccharomyces cerevisiae
dc.titlePGU1 gene natural deletion is responsible for the absence of endo-polygalacturonase activity in some wine strains of Saccharomyces cerevisiae
dc.typeArticle
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