Mitochondrial Acyl-CoA, adenine nucleotide translocase activity and oxidative phosphorylation in myocardial ischaemia

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dc.contributor.authorLochner A.
dc.contributor.authorVan Niekerk I.
dc.contributor.authorKotze J.C.N.
dc.date.accessioned2011-05-15T15:59:44Z
dc.date.available2011-05-15T15:59:44Z
dc.date.issued1981
dc.description.abstractThe hypothesis that inhibition of mitochondrial adenine nucleotide translocase activity by accumulation of long chain acyl-CoA esters might be responsible for the depression in mitochondrial energy metabolism in myocardial ischaemia was reinvestigated. Hypoxic, low flow perfusion (substrate free) of the isolated rat heart resulted in increases in tissue and mitochondrial acyl-CoA contents together with a reduction in adenine nucleotide translocase activity and oxidative phosphorylation function of mitochondria isolated from the tissue. Addition of exogenous substrates together with insulin or propranolol to the perfusate (glucose and insulin; glucose, glycerol and insulin; glucose, propranolol and insulin) caused significant further increases in tissue and mitochondrial acyl-CoA contents. In contrast, the mitochondrial adenine nucleotide translocase activity and oxidative phosphorylation function improved and were similar to those of control perfused hearts. The difference between the in vivo and in vitro effects of acyl-CoA on mitochondrial adenine and nucleotide translocase and oxidative phosphorylation could be due to in vivo binding to intracellular fatty acid binding proteins.
dc.description.versionArticle
dc.identifier.citationJournal of Molecular and Cellular Cardiology
dc.identifier.citation13
dc.identifier.citation11
dc.identifier.issn222828
dc.identifier.urihttp://hdl.handle.net/10019.1/11335
dc.subjectacyl coenzyme a
dc.subjectadenine nucleotide translocase
dc.subjectenzyme
dc.subjectpotassium cyanide
dc.subjectradioisotope
dc.subjectadenosine triphosphate c 14
dc.subjectanimal experiment
dc.subjectheart
dc.subjectheart mitochondrion
dc.subjectheart muscle
dc.subjectheart muscle ischemia
dc.subjectischemia
dc.subjectmitochondrion
dc.subjectoxidative phosphorylation
dc.subjectrat
dc.subjectregional perfusion
dc.subjectAcyl Coenzyme A
dc.subjectAnimal
dc.subjectCoronary Disease
dc.subjectFemale
dc.subjectHeart
dc.subjectInsulin
dc.subjectMitochondria, Heart
dc.subjectMitochondrial ADP, ATP Translocases
dc.subjectNucleotidyltransferases
dc.subjectOxidative Phosphorylation
dc.subjectOxygen Consumption
dc.subjectPerfusion
dc.subjectPropranolol
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.titleMitochondrial Acyl-CoA, adenine nucleotide translocase activity and oxidative phosphorylation in myocardial ischaemia
dc.typeArticle
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