Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: Three carnitine acetyltransferases are essential in a carnitine-dependent strain

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dc.contributor.authorSwiegers, J. H.en_ZA
dc.contributor.authorDippenaar, N.en_ZA
dc.contributor.authorPretorius, I. S.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T16:05:34Z
dc.date.available2011-05-15T16:05:34Z
dc.date.issued2001
dc.description.abstractL-Carnitine is required for the transfer of activated acyl-groups across intracellular membranes in eukaryotic organisms. In Saccharomyces cerevisiae, peroxisomal membranes are impermeable to acetyl-CoA, which is produced in the peroxisome when cells are grown on fatty acids as carbon source. In a reversible reaction catalysed by carnitine acetyltransferases (CATs), activated acetyl groups are transferred to carnitine to form acetylcarnitine which can be shuttled across membranes. Here we describe a mutant selection strategy that specifically selects for mutants affected in carnitine-dependent metabolic activities. Complementation of three of these mutants resulted in the cloning of three CAT encoding genes: CAT2, coding for the carnitine acetyltransferase associated with the peroxisomes and the mitochondria; YAT1, coding for the carnitine acetyltransferase, which is presumably associated with the outer mitochondrial membrane, and YER024w (YAT2), which encodes a third, previously unidentified carnitine acetyltransferase. The data also show that (a) L-carnitine and all three CATs are essential for growth on non-fermentable carbon sources in a strain with a disrupted CIT2 gene; (b) Yat2p contributes significantly to total CAT activity when cells are grown on ethanol; and that (c) the carnitine-dependent transfer of activated acetyl groups plays a more important role in cellular processes than previously realised. Copyright © 2001 John Wiley & Sons, Ltd.en_ZA
dc.description.versionArticle
dc.identifier.citationYeast
dc.identifier.citation18
dc.identifier.citation7
dc.identifier.issn0749503X
dc.identifier.other10.1002/yea.712
dc.identifier.urihttp://hdl.handle.net/10019.1/13193
dc.subjectacetyl coenzyme A
dc.subjectacetylcarnitine
dc.subjectcarbon
dc.subjectcarnitine
dc.subjectcarnitine acetyltransferase
dc.subjectfatty acid
dc.subjectarticle
dc.subjectcatalysis
dc.subjectcell growth
dc.subjectcell membrane transport
dc.subjectchemical reaction
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjecteukaryote
dc.subjectfermentation
dc.subjectfungus growth
dc.subjectfungus mutant
dc.subjectgene disruption
dc.subjectgenetic complementation
dc.subjectintracellular membrane
dc.subjectmetabolic activation
dc.subjectmitochondrial membrane
dc.subjectmitochondrion
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectouter membrane
dc.subjectperoxisome
dc.subjectpriority journal
dc.subjectSaccharomyces cerevisiae
dc.subjectCarnitine
dc.subjectCarnitine Acyltransferases
dc.subjectCarnitine O-Acetyltransferase
dc.subjectCloning, Molecular
dc.subjectDNA, Fungal
dc.subjectEthanol
dc.subjectGenetic Complementation Test
dc.subjectMembrane Proteins
dc.subjectMutagenesis
dc.subjectPlasmids
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSequence Analysis, DNA
dc.subjectEukaryota
dc.subjectFelis catus
dc.subjectSaccharomyces cerevisiae
dc.titleCarnitine-dependent metabolic activities in Saccharomyces cerevisiae: Three carnitine acetyltransferases are essential in a carnitine-dependent strain
dc.typeArticle
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