Implications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae

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dc.contributor.authorToh T.-H.
dc.contributor.authorKayingo G.
dc.contributor.authorVan Der Merwe M.J.
dc.contributor.authorKilian S.G.
dc.contributor.authorHallsworth J.E.
dc.contributor.authorHohmann S.
dc.contributor.authorPrior B.A.
dc.date.accessioned2011-05-15T16:03:28Z
dc.date.available2011-05-15T16:03:28Z
dc.date.issued2001
dc.description.abstractThe deletion of the gene encoding the glycerol facilitator Fps1p was associated with an altered plasma membrane lipid composition in Saccharomyces cerevisiae. The S. cerevisiae fps1Δ strain respectively contained 18 and 26% less ergosterol than the wild-type strain, at the whole-cell level and at the plasma membrane level. Other mutants with deficiencies in glycerol metabolism were studied to investigate any possible link between membrane ergosterol content and intracellular glycerol accumulation. In these mutants a modification in intracellular glycerol concentration, or in intra- to extracellular glycerol ratio was accompanied by a reduction in plasma membrane ergosterol content. However, there was no direct correlation between ergosterol content and intracellular glycerol concentration. Lipid composition influences the membrane permeability for solutes during adaptation of yeast cells to osmotic stress. In this study, ergosterol supplementation was shown to partially suppress the hypo-osmotic sensitivity phenotype of the fps1Δ strain, leading to more efficient glycerol efflux, and improved survival. The erg-1 disruption mutant, which is unable to synthesise ergosterol, survived and recovered from the hypo-osmotic shock more successfully when the concentration of exogenously supplied ergosterol was increased. The results obtained suggest that a higher ergosterol content facilitates the flux of glycerol across the plasma membrane of S. cerevisiae cells. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation1
dc.identifier.citation3
dc.identifier.issn15671356
dc.identifier.other10.1016/S1567-1356(01)00032-0
dc.identifier.urihttp://hdl.handle.net/10019.1/12631
dc.subjectergosterol
dc.subjectglycerol
dc.subjectFPS1 protein, S cerevisiae
dc.subjectmembrane protein
dc.subjectSaccharomyces cerevisiae protein
dc.subjectarticle
dc.subjectcell membrane
dc.subjectcell membrane transport
dc.subjectcell survival
dc.subjectcontrolled study
dc.subjectgene deletion
dc.subjectlipid composition
dc.subjectlipid transport
dc.subjectlipogenesis
dc.subjectmutant
dc.subjectnonhuman
dc.subjectosmosis
dc.subjectosmotic stress
dc.subjectphenotype
dc.subjectSaccharomyces cerevisiae
dc.subjectyeast cell
dc.subjectcell membrane permeability
dc.subjectculture medium
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectosmotic pressure
dc.subjectphysiology
dc.subjecttransport at the cellular level
dc.subjectSaccharomyces
dc.subjectSaccharomyces cerevisiae
dc.subjectBiological Transport
dc.subjectCell Membrane
dc.subjectCell Membrane Permeability
dc.subjectCulture Media
dc.subjectErgosterol
dc.subjectGene Deletion
dc.subjectGlycerol
dc.subjectMembrane Proteins
dc.subjectOsmotic Pressure
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.titleImplications of FPS1 deletion and membrane ergosterol content for glycerol efflux from Saccharomyces cerevisiae
dc.typeArticle
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