Exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk

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dc.contributor.authorBauer R.
dc.contributor.authorBekker J.P.
dc.contributor.authorWyk N.v.
dc.contributor.authordu Toit C.
dc.contributor.authorDicks L.M.T.
dc.contributor.authorKossmann J.
dc.date.accessioned2011-05-15T15:56:47Z
dc.date.available2011-05-15T15:56:47Z
dc.date.issued2009
dc.description.abstractWith increasing consumer demands for safer, healthier and more natural products, bacterially produced exopolysaccharides (EPSs) are becoming a viable option as an additive in designer-type foods. Fresh milk samples from cattle and sheep were collected from informal settlements in South Africa. After a three day incubation period at 25 °C, 550 bacterial strains were isolated and evaluated for EPS production from lactose as sole carbon source. Strains producing EPS on lactose were identified to species level with 16S rRNA gene sequencing and encompass 11 Gram-positive and 6 Gram-negative bacteria. EPS production was assigned for the first time to members of the species Staphylococcus hominis and Enterococcus lactis, and also to apparently novel species of the genera Sphingomonas and Acinetobacter. The polymers consisted mainly out of galactose and glucose, while a few isolates also incorporated rhamnose. Isolates produced diverse biopolymers as seen by significant differences in monomer ratios. © 2009 Elsevier B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation131
dc.identifier.citation03-Feb
dc.identifier.issn1681605
dc.identifier.other10.1016/j.ijfoodmicro.2009.02.020
dc.identifier.urihttp://hdl.handle.net/10019.1/10037
dc.subjectexopolysaccharide
dc.subjectgalactose
dc.subjectglucose
dc.subjectlactose
dc.subjectrhamnose
dc.subjectRNA 16S
dc.subjectAcinetobacter
dc.subjectAcinetobacter johnsonii
dc.subjectarticle
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterium identification
dc.subjectbacterium isolation
dc.subjectcarbon source
dc.subjectComamonas
dc.subjectComamonas denitrificans
dc.subjectcontrolled study
dc.subjectcow
dc.subjectEnterobacter aerogenes
dc.subjectEnterococcus
dc.subjectEnterococcus lactis
dc.subjectEscherichia coli
dc.subjectfermentation
dc.subjectgene sequence
dc.subjectGram negative bacterium
dc.subjectGram positive bacterium
dc.subjectincubation temperature
dc.subjectincubation time
dc.subjectmilk
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectPropionibacterium acnes
dc.subjectsheep
dc.subjectSphingomonas
dc.subjectSphingomonas echinoides
dc.subjectStaphylococcus hominis
dc.subjectAcinetobacter
dc.subjectAnimals
dc.subjectBacteria
dc.subjectBase Sequence
dc.subjectCattle
dc.subjectEnterococcus
dc.subjectFermentation
dc.subjectHexoses
dc.subjectHydrolysis
dc.subjectLactose
dc.subjectMilk
dc.subjectPolysaccharides, Bacterial
dc.subjectRNA, Ribosomal
dc.subjectSequence Homology
dc.subjectSheep
dc.subjectSouth Africa
dc.subjectSphingomonas
dc.subjectStaphylococcus hominis
dc.subjectAcinetobacter
dc.subjectBacteria (microorganisms)
dc.subjectBos
dc.subjectEnterococcus lactis
dc.subjectNegibacteria
dc.subjectOvis aries
dc.subjectPosibacteria
dc.subjectSphingomonas
dc.subjectStaphylococcus hominis
dc.titleExopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk
dc.typeArticle
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