Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization

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dc.contributor.authorSmith A.S.G.
dc.contributor.authorRawlings D.E.
dc.date.accessioned2011-05-15T16:01:53Z
dc.date.available2011-05-15T16:01:53Z
dc.date.issued1998
dc.description.abstractThe pasABC genes of the proteic plasmid addiction system of broad-host- range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100- fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-β-D-thiogalactopyranoside (IPTG)- regulated tac promoter, they were unable to stabilize a heterologous test plasmid.
dc.description.versionArticle
dc.identifier.citationJournal of Bacteriology
dc.identifier.citation180
dc.identifier.citation20
dc.identifier.issn219193
dc.identifier.urihttp://hdl.handle.net/10019.1/12202
dc.subjectAcidithiobacillus ferrooxidans
dc.subjectarticle
dc.subjectbacterial genetics
dc.subjectgene control
dc.subjectgene expression
dc.subjectgene expression regulation
dc.subjectgene structure
dc.subjectnonhuman
dc.subjectplasmid
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectAntidotes
dc.subjectBacterial Proteins
dc.subjectBacterial Toxins
dc.subjectEscherichia coli
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenes, Reporter
dc.subjectPlasmids
dc.subjectPromoter Regions (Genetics)
dc.subjectAcidithiobacillus ferrooxidans
dc.subjectBacteria (microorganisms)
dc.subjectNegibacteria
dc.subjectThiobacillus
dc.titleAutoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization
dc.typeArticle
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