Effect of conjugated linoleic acid on testosterone levels in vitro and in vivo after an acute bout of resistance exercise

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dc.contributor.authorMacAluso F.
dc.contributor.authorMorici G.
dc.contributor.authorCatanese P.
dc.contributor.authorArdizzone N.M.
dc.contributor.authorGammazza A.M.
dc.contributor.authorBonsignore G.
dc.contributor.authorGiudice G.L.
dc.contributor.authorStampone T.
dc.contributor.authorBarone R.
dc.contributor.authorFarina F.
dc.contributor.authorDi Felice V.
dc.date.accessioned2012-07-26T07:16:18Z
dc.date.available2012-07-26T07:16:18Z
dc.date.issued2012
dc.descriptionArticle
dc.description.abstractThe purposes of the present study were to investigate the effect of conjugated linoleic acid (CLA) supplementation on testosterone levels in vitro on a cell line derived from Leydig cells (R2C) and in vivo in the blood of physically active subjects before and after a resistance exercise bout. In vitro R2C cells were treated with different CLA concentrations (0-30 μM) for 24 and 48 hours. After treatment, supernatant media were tested to determine testosterone secretion. The CLA increased the testosterone secretion only after 48 hours. In vivo, 10 resistance-trained male subjects, in a double-blind placebo-controlled and crossover study design were randomized for 3 weeks of either ± g d -1 CLA or placebo. Blood was drawn pre and post each resistance exercise bout to determine the total testosterone and sex hormone-binding globulin (SHBG) levels. No significant differences were observed for total testosterone or SHBG pre and post each resistance exercise bout; although after the resistance exercise bouts, total testosterone increased moderately (effect size = moderate), whereas after CLA supplementation, there was a large increase in total testosterone (effect size = large). CLA supplementation induced an increase in testosterone levels in Leydig cells in vitro after 48 hours but not in vivo before and after a resistance exercise bout. These findings suggest that CLA supplementation may promote testosterone synthesis through a molecular pathway that should be investigated in the future, although this effect did not have an anabolic relevance in our in vivo model. © 2012 National Strength and Conditioning Association.
dc.identifier.citationJournal of Strength and Conditioning Research
dc.identifier.citation26
dc.identifier.citation6
dc.identifier.citation1667
dc.identifier.citation1674
dc.identifier.issn10648011
dc.identifier.otherdoi:10.1519/JSC.0b013e318231ab78
dc.identifier.urihttp://hdl.handle.net/10019.1/21847
dc.subjectBody composition
dc.subjectFood supplement
dc.subjectHormones
dc.subjectLeydig cell
dc.titleEffect of conjugated linoleic acid on testosterone levels in vitro and in vivo after an acute bout of resistance exercise
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