Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model
dc.contributor.author | Pluddemann A. | |
dc.contributor.author | van Zyl W.H. | |
dc.date.accessioned | 2011-05-15T16:01:28Z | |
dc.date.available | 2011-05-15T16:01:28Z | |
dc.date.issued | 2003 | |
dc.description.abstract | The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene. Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system. | |
dc.description.version | Article | |
dc.identifier.citation | Current Genetics | |
dc.identifier.citation | 43 | |
dc.identifier.citation | 6 | |
dc.identifier.issn | 1728083 | |
dc.identifier.other | 10.1007/s00294-003-0409-0 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/11998 | |
dc.subject | dimer | |
dc.subject | glyceraldehyde 3 phosphate dehydrogenase | |
dc.subject | hepatitis B surface antigen | |
dc.subject | recombinant protein | |
dc.subject | virus envelope protein | |
dc.subject | vitronectin | |
dc.subject | article | |
dc.subject | Aspergillus nidulans | |
dc.subject | Aspergillus niger | |
dc.subject | cost effectiveness analysis | |
dc.subject | electron microscopy | |
dc.subject | fungal strain | |
dc.subject | fungus culture | |
dc.subject | gene cassette | |
dc.subject | gene expression | |
dc.subject | genetic transcription | |
dc.subject | genetic transformation | |
dc.subject | genome | |
dc.subject | Hepatitis B virus | |
dc.subject | host | |
dc.subject | nonhuman | |
dc.subject | particle size | |
dc.subject | priority journal | |
dc.subject | promoter region | |
dc.subject | protein purification | |
dc.subject | protein synthesis | |
dc.subject | vaccine production | |
dc.subject | virus gene | |
dc.subject | virus particle | |
dc.subject | Western blotting | |
dc.subject | Aspergillus niger | |
dc.subject | Bioreactors | |
dc.subject | Biotechnology | |
dc.subject | Gene Dosage | |
dc.subject | Genetic Engineering | |
dc.subject | Hepatitis B Surface Antigens | |
dc.subject | Hepatitis B virus | |
dc.subject | Humans | |
dc.subject | Models, Genetic | |
dc.subject | Promoter Regions (Genetics) | |
dc.subject | Transformation, Genetic | |
dc.subject | Transgenes | |
dc.subject | Viral Proteins | |
dc.subject | Virion | |
dc.subject | Aspergillus | |
dc.subject | Aspergillus niger | |
dc.subject | Emericella nidulans | |
dc.subject | Fungi | |
dc.subject | Hepatitis B virus | |
dc.title | Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model | |
dc.type | Article |