Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes

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dc.contributor.authorTraff K.L.
dc.contributor.authorCordero R.R.O.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorHahn-Hagerdal B.
dc.date.accessioned2011-05-15T15:56:33Z
dc.date.available2011-05-15T15:56:33Z
dc.date.issued2001
dc.description.abstractSaccharomyces cerevisiae ferments hexoses efficiently but is unable to ferment xylose. When the bacterial enzyme xylose isomerase (XI) from Thermus thermophilus was produced in S. cerevisiae, xylose utilization and ethanol formation were demonstrated. In addition, xylitol and acetate were formed. An unspecific aldose reductase (AR) capable of reducing xylose to xylitol has been identified in S. cerevisiae. The GRE3 gene, encoding the AR enzyme, was deleted in S. cerevisiae CEN.PK2-1C, yielding YUSM1009a. XI from T. thermophilus was produced, and endogenous xylulokinase from S. cerevisiae was overproduced in S. cerevisiae CEN.PK2-1C and YUSM1009a. In recombinant strains from which the GRE3 gene was deleted, xylitol formation decreased twofold. Deletion of the GRE3 gene combined with expression of the xylA gene from T. thermophilus on a replicative plasmid generated recombinant xylose utilizing S. cerevisiae strain TMB3102, which produced ethanol from xylose with a yield of 0.28 mmol of C from ethanol/mmol of C from xylose. None of the recombinant strains grew on xylose.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation67
dc.identifier.citation12
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.67.12.5668-5674.2001
dc.identifier.urihttp://hdl.handle.net/10019.1/9915
dc.subjectaldehyde reductase
dc.subjectAldose Ketose Isomerases
dc.subjectglucose
dc.subjectisomerase
dc.subjectoxygen
dc.subjectphosphotransferase
dc.subjectxylose
dc.subjectxylose isomerase
dc.subjectxylulokinase
dc.subjectanaerobic growth
dc.subjectarticle
dc.subjectenzymology
dc.subjectgene deletion
dc.subjectgenetic recombination
dc.subjectgenetics
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectSaccharomyces cerevisiae
dc.subjectAldehyde Reductase
dc.subjectAldose-Ketose Isomerases
dc.subjectAnaerobiosis
dc.subjectGene Deletion
dc.subjectGlucose
dc.subjectOxygen
dc.subjectPhosphotransferases (Alcohol Group Acceptor)
dc.subjectRecombination, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectSupport, Non-U.S. Gov't
dc.subjectXylose
dc.titleDeletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing the xylA and XKS1 Genes
dc.typeArticle
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