Expression of the immunity protein of plantaricin 423, produced by Lactobacillus plantarum 423, and analysis of the plasmid encoding the bacteriocin

Simple item page
dc.contributor.authorVan Reenen C.A.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:32Z
dc.date.available2011-05-15T15:56:32Z
dc.date.issued2006
dc.description.abstractPlantaricin 423 is a class IIa bacteriocin produced by Lactobacillus plantarum isolated from sorghum beer. It has been previously determined that plantaricin 423 is encoded by a plasmid designated pPLA4, which is now completely sequenced. The plantaricin 423 operon shares high sequence similarity with the operons of coagulin, pediocin PA-1, and pediocin AcH, with small differences in the DNA sequence encoding the mature bacteriocin peptide and the immunity protein. Apart from the bacteriocin operon, no significant sequence similarity could be detected between the DNA or translated sequence of pPLA4 and the available DNA or translated sequences of the plasmids encoding pediocin AcH, pediocin PA-1, and coagulin, possibly indicating a different origin. In addition to the bacteriocin operon, sequence analysis of pPLA4 revealed the presence of two open reading frames (ORFs). ORF1 encodes a putative mobilization (Mob) protein that is homologous to the pMV158 superfamily of mobilization proteins. Highest sequence similarity occurred between this protein and the Mob protein of L. plantarum NCDO 1088. ORF2 encodes a putative replication protein that revealed low sequence similarity to replication proteins of plasmids pLME300 from Lactobacillus fermentum and pYIT356 from Lactobacillus casei. The immunity protein of plantaricin 423 contains 109 amino acids. Although plantaricin 423 shares high sequence similarity with the pediocin PA-1 operon, no cross-reactivity was recorded between the immunity proteins of plantaricin 423 and pediocin PA-1. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation72
dc.identifier.citation12
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.01428-06
dc.identifier.urihttp://hdl.handle.net/10019.1/9901
dc.subjectAmino acids
dc.subjectBacteria
dc.subjectDNA
dc.subjectImmunization
dc.subjectMicrobiology
dc.subjectImmunity protein
dc.subjectLactobacillus plantarum
dc.subjectOpen reading frames (ORF)
dc.subjectPlasmid encoding
dc.subjectProteins
dc.subjectamino acid
dc.subjectbacterial protein
dc.subjectbacteriocin
dc.subjectcoagulin
dc.subjectDNA
dc.subjectpediocin ach
dc.subjectpediocin PA 1
dc.subjectplantaricin 423
dc.subjectprotein Mob
dc.subjectbacterium
dc.subjectDNA
dc.subjectgenetic analysis
dc.subjectimmunity
dc.subjectisolated population
dc.subjectplasmid
dc.subjectprotein
dc.subjectamino acid sequence
dc.subjectarticle
dc.subjectbeer
dc.subjectcontrolled study
dc.subjectcross reaction
dc.subjectDNA replication origin
dc.subjectDNA sequence
dc.subjectgene sequence
dc.subjectLactobacillus casei
dc.subjectLactobacillus fermentum
dc.subjectLactobacillus plantarum
dc.subjectLactobacillus plantarum 423
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectopen reading frame
dc.subjectoperon
dc.subjectplasmid
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein family
dc.subjectprotein isolation
dc.subjectproteomics
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectsequence homology
dc.subjectsorghum
dc.subjectAmino Acid Sequence
dc.subjectBacterial Proteins
dc.subjectBacteriocins
dc.subjectBase Sequence
dc.subjectDrug Resistance, Bacterial
dc.subjectLactobacillus plantarum
dc.subjectMicrobial Sensitivity Tests
dc.subjectMolecular Sequence Data
dc.subjectOperon
dc.subjectPlasmids
dc.subjectSequence Analysis, DNA
dc.subjectLactobacillus casei
dc.subjectLactobacillus fermentum
dc.subjectLactobacillus plantarum
dc.titleExpression of the immunity protein of plantaricin 423, produced by Lactobacillus plantarum 423, and analysis of the plasmid encoding the bacteriocin
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications