Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus

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dc.contributor.authorVan Zyl L.J.
dc.contributor.authorDeane S.M.
dc.contributor.authorLouw L.-A.
dc.contributor.authorRawlings D.E.
dc.date.accessioned2011-05-15T15:56:31Z
dc.date.available2011-05-15T15:56:31Z
dc.date.issued2008
dc.description.abstractThree large cryptic plasmids from different isolates of Acidithiobacillus caldus were rescued by using an in vitro transposition system that delivers a kanamycin-selectable marker and an Escherichia coli plasmid origin of replication. The largest of the plasmids, the 65-kb plasmid pTcM1, was isolated from a South African A. caldus strain, MNG. This plasmid was sequenced and compared to that of pTcF1 (39 kb, from strain "f," South Africa) and pC-SH12 (29 kb, from strain C-SH12, Australia). With the exception of a 2.7-kb insertion sequence, pC-SH12 appears to represent the DNA common to all three plasmids and includes a number of accessory genes plus the plasmid "backbone" containing the replication region. The two larger plasmids carry, in addition, a number of insertion sequences of the ISL3 family and a composite transposon related to the Tn21 subfamily containing a highly mosaic region within the borders of the inverted repeats. Genes coding for arsenic resistance, plasmid mobilization, plasmid stability, and a putative restriction-modification system occur within these mosaic regions. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation74
dc.identifier.citation14
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.00864-08
dc.identifier.urihttp://hdl.handle.net/10019.1/9899
dc.subjectArsenic
dc.subjectEscherichia coli
dc.subjectGene encoding
dc.subjectGenes
dc.subjectNonmetals
dc.subjectNucleic acids
dc.subjectOrganic acids
dc.subjectProgramming theory
dc.subjectStrain
dc.subjectSulfur
dc.subjectSystem stability
dc.subjectAcidithiobacillus caldus
dc.subjectAmerican society
dc.subjectArsenic resistance
dc.subjectAustralia
dc.subjectCryptic plasmids
dc.subjectGenes coding
dc.subjectIn-vitro
dc.subjectInsertion sequence (IS)
dc.subjectKanamycin (KM)
dc.subjectPlasmid stability
dc.subjectRestriction modification
dc.subjectSouth Africa (SA)
dc.subjectSulfur oxidizing bacterium (SOB)
dc.subjectHealth
dc.subjectDNA fragment
dc.subjectarsenic
dc.subjectbacterium
dc.subjectbiomarker
dc.subjectcomparative study
dc.subjectDNA
dc.subjectgene expression
dc.subjectoxidation
dc.subjectplasmid
dc.subjectsulfur
dc.subjectAcidithiobacillus caldus
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectcontrolled study
dc.subjectgene sequence
dc.subjectgene structure
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectplasmid
dc.subjectreplicon
dc.subjectspecies difference
dc.subjectsulfur oxidizing bacterium
dc.subjecttransposon
dc.subjectAcidithiobacillus
dc.subjectAustralia
dc.subjectChromosome Mapping
dc.subjectCloning, Molecular
dc.subjectDNA Transposable Elements
dc.subjectDNA, Bacterial
dc.subjectOpen Reading Frames
dc.subjectPlasmids
dc.subjectReplicon
dc.subjectRestriction Mapping
dc.subjectSequence Analysis, DNA
dc.subjectSouth Africa
dc.subjectSulfur-Reducing Bacteria
dc.subjectAcidithiobacillus caldus
dc.subjectBacteria (microorganisms)
dc.subjectEscherichia coli
dc.titlePresence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus
dc.typeArticle
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