Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. Dominates south African commercial biooxidation tanks that operate at 40°C

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dc.contributor.authorCoram N.J.
dc.contributor.authorRawlings D.E.
dc.date.accessioned2011-05-15T15:56:33Z
dc.date.available2011-05-15T15:56:33Z
dc.date.issued2002
dc.description.abstractIron-oxidizing bacteria belonging to the genus Leptospirillum are of great importance in continuous-flow commercial biooxidation reactors, used for extracting metals from minerals, that operate at 40°C or less. They also form part of the microbial community responsible for the generation of acid mine drainage. More than 16 isolates of leptospirilla were included in this study, and they were clearly divisible into two major groups. Group I leptospirilla had G+C moles percent ratios within the range 49 to 52% and had three copies of rrn genes, and based on 16S rRNA sequence data, these isolates clustered together with the Leptospirillum ferrooxidans type strain (DSM2705 or L15). Group II leptospirilla had G+C moles percent ratios of 55 to 58% and had two copies of rrn genes, and based on 16S rRNA sequence data, they form a separate cluster. Genome DNA-DNA hybridization experiments indicated that three similarity subgroups were present among the leptospirilla tested, with two DNA-DNA hybridization similarity subgroups found within group I. The two groups could also be distinguished based on the sizes of their 16S-23S rRNA gene spacer regions. We propose that the group II leptospirilla should be recognized as a separate species with the name Leptospirillum ferriphilum sp. nov. Members of the two species can be rapidly distinguished from each other by amplification of their 16S rRNA genes and by carrying out restriction enzyme digests of the products. Several, but not all, isolates of the group II leptospirilla, but none from group I (L. ferrooxidans), were capable of growth at 45°C. All the leptospirilla isolated from commercial biooxidation tanks in South Africa were from group II.
dc.description.versionArticle
dc.identifier.citationApplied and Environmental Microbiology
dc.identifier.citation68
dc.identifier.citation2
dc.identifier.issn992240
dc.identifier.other10.1128/AEM.68.2.838-845.2002
dc.identifier.urihttp://hdl.handle.net/10019.1/9914
dc.subjectBacteria
dc.subjectDNA
dc.subjectEnzymes
dc.subjectGenes
dc.subjectMinerals
dc.subjectRNA
dc.subjectBiooxidation
dc.subjectMicrobiology
dc.subjectbacterial RNA
dc.subjectribosome RNA
dc.subjectRNA 16S
dc.subjectspacer DNA
dc.subjectbioreactor
dc.subjectarticle
dc.subjectbacterial genetics
dc.subjectbacterium
dc.subjectbioreactor
dc.subjectDNA DNA hybridization
dc.subjectgene amplification
dc.subjectgenus
dc.subjectleptospirillum ferriphilum
dc.subjectleptospirillum ferrooxidans
dc.subjectmolecular biology
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectoxidation
dc.subjectRNA sequence
dc.subjectSouth Africa
dc.subjectspecies
dc.subjectBioreactors
dc.subjectDNA, Ribosomal
dc.subjectGenes, rRNA
dc.subjectGram-Negative Bacteria
dc.subjectIron
dc.subjectMining
dc.subjectMolecular Sequence Data
dc.subjectNucleic Acid Hybridization
dc.subjectOxidation-Reduction
dc.subjectRibotyping
dc.subjectRNA, Ribosomal, 16S
dc.subjectSequence Analysis, DNA
dc.subjectSouth Africa
dc.subjectBacteria (microorganisms)
dc.subjectLeptospirillum
dc.subjectLeptospirillum ferriphilum
dc.subjectLeptospirillum ferrooxidans
dc.titleMolecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. Dominates south African commercial biooxidation tanks that operate at 40°C
dc.typeArticle
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