Effect of dimorphic regulation on heterologous glucose oxidase production by Mucor circinelloides

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dc.contributor.authorBredenkamp A.
dc.contributor.authorVelankar H.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorGorgens J.F.
dc.date.accessioned2011-05-15T16:05:33Z
dc.date.available2011-05-15T16:05:33Z
dc.date.issued2010
dc.description.abstractThe production of Aspergillus niger glucose oxidase (GOX) and native amylase by the recombinant M. circinelloides KFA199 strain under conditions of dimorphic growth was investigated. The recombinant KFA199 strain was compared to its parental ATCC 1216b strain and a wild-type CBS 232.29 strain under similar morphology-controlled conditions. Cultivation in Vogel's medium supplemented with ergosterol/Tween-80 and sparged with nitrogen gas was most suitable for yeast-like biomass production under anaerobic conditions. Anaerobic growth was characterized by high levels of ethanol formation and linear growth rates of 0.24-0.05/h, indicating metabolic stress. Subsequent to anaerobic growth, cultures were shifted to aerobic conditions to induce aerobic mycelial growth. GOX produced by the recombinant KFA199 after the shift to aerobic conditions was poorly secreted and accumulated intracellularly to 0.56 U/mlculture. Amylase production by the KFA199, ATCC12b and CBS 232.29 strains was determined during growth on starch after the shift to aerobic culture. Growth-associated amylase production by the ATCC 1216b (0.63 U/mlculture) and wild-type CBS 232.29 (0.33 U/mlculture) strains was substantially higher than by the recombinant KFA199 strain (0.07 U/mlculture), which may be related to the leucine auxotrophy of the transformation host, or genetic changes induced during transformation of the KFA199 strain. © 2010 John Wiley & Sons, Ltd.
dc.description.versionArticle
dc.identifier.citationYeast
dc.identifier.citation27
dc.identifier.citation10
dc.identifier.issn0749503X
dc.identifier.other10.1002/yea.1793
dc.identifier.urihttp://hdl.handle.net/10019.1/13186
dc.subjectalcohol
dc.subjectamylase
dc.subjectergosterol
dc.subjectglucose oxidase
dc.subjectnitrogen
dc.subjectpolysorbate 80
dc.subjectanaerobic growth
dc.subjectarticle
dc.subjectbiomass production
dc.subjectcoculture
dc.subjectculture medium
dc.subjectfungal strain
dc.subjectfungus culture
dc.subjectfungus growth
dc.subjectmetabolic stress
dc.subjectmorphology
dc.subjectMucor
dc.subjectmucor circinelloides
dc.subjectmycelial growth
dc.subjectpriority journal
dc.subjectwild type
dc.subjectAerobiosis
dc.subjectAmylases
dc.subjectAnaerobiosis
dc.subjectAspergillus niger
dc.subjectBiomass
dc.subjectBioreactors
dc.subjectBiotechnology
dc.subjectCulture Media
dc.subjectFermentation
dc.subjectGene Expression Regulation, Fungal
dc.subjectGlucose Oxidase
dc.subjectMucor
dc.subjectOxygen
dc.subjectRecombinant Proteins
dc.subjectStarch
dc.subjectAspergillus niger
dc.subjectFungi
dc.subjectMucor circinelloides
dc.titleEffect of dimorphic regulation on heterologous glucose oxidase production by Mucor circinelloides
dc.typeArticle
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