Regulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2

Simple item page
dc.contributor.authorSwiegers, J. H.en_ZA
dc.contributor.authorPretorius, I. S.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T16:01:28Z
dc.date.available2011-05-15T16:01:28Z
dc.date.issued2006
dc.description.abstractIn Saccharomyces cerevisiae the Ras/cAMP/PKA signalling pathway controls multiple metabolic pathways, and alterations in the intracellular concentrations of cAMP through modification of signalling pathway factors can be lethal or result in severe growth defects. In this work, the important role of Ras2p in metabolic regulation during growth on the non-fermentable carbon source glycerol is further investigated. The data show that the overexpression of RAS2 suppresses the growth defect of the glyoxylate cycle citrate synthase mutant, cit2Δ. The overexpression results in enhanced proliferation and biomass yield when cells are grown on glycerol as sole carbon source, and increases citrate synthase activity and intracellular citrate concentration. Interestingly, the suppression of cit2Δ and the enhanced proliferation and biomass yield are only observed when RAS2 is overexpressed and not in strains containing the constitutively active allele RAS2 val19. However, both RAS2 and RAS2 val19upregulated citrate synthase activity. We propose that the RAS2 overexpression results in a combination of general upregulation of respiratory growth capacity and an increase in mitochondrial citrate/ citrate synthases, which together, complement the metabolic requirements of the cit2Δ mutant. The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source. © Springer-Verlag 2006.
dc.description.versionArticle
dc.identifier.citationCurrent Genetics
dc.identifier.citation50
dc.identifier.citation3
dc.identifier.issn1728083
dc.identifier.other10.1007/s00294-006-0084-z
dc.identifier.urihttp://hdl.handle.net/10019.1/11995
dc.subjectcarbon
dc.subjectcitrate synthase
dc.subjectcitric acid
dc.subjectcomplementary DNA
dc.subjectcyclic AMP
dc.subjectcyclic AMP dependent protein kinase
dc.subjectfungal DNA
dc.subjectglycerol
dc.subjectglyoxylic acid
dc.subjectmutant protein
dc.subjectRas protein
dc.subjectRas2 protein
dc.subjectSaccharomyces cerevisiae protein
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectcell growth
dc.subjectcell level
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectenzyme activity
dc.subjectgene overexpression
dc.subjectlethality
dc.subjectmetabolic regulation
dc.subjectmicrobial biomass
dc.subjectmitochondrial respiration
dc.subjectmolecular cloning
dc.subjectNeurospora crassa
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectregulatory mechanism
dc.subjectSaccharomyces cerevisiae
dc.subjectsignal transduction
dc.subjectstrain difference
dc.subjectupregulation
dc.subjectBase Sequence
dc.subjectBiomass
dc.subjectCell Proliferation
dc.subjectCitrate (si)-Synthase
dc.subjectCitric Acid
dc.subjectCloning, Molecular
dc.subjectCyclic AMP
dc.subjectCyclic AMP-Dependent Protein Kinases
dc.subjectDNA, Fungal
dc.subjectGene Expression
dc.subjectGenes, Fungal
dc.subjectGlycerol
dc.subjectGlyoxylates
dc.subjectMAP Kinase Signaling System
dc.subjectMitochondria
dc.subjectMutation
dc.subjectras Proteins
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSignal Transduction
dc.subjectSuppression, Genetic
dc.subjectSaccharomyces cerevisiae
dc.titleRegulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications