Biofilm-forming capacity, surface hydrophobicity and aggregation characteristics of Myroides odoratus isolated from South African Oreochromis mossambicus fish

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dc.contributor.authorJacobs A.
dc.contributor.authorChenia H.Y.
dc.date.accessioned2011-05-15T15:59:23Z
dc.date.available2011-05-15T15:59:23Z
dc.date.issued2009
dc.description.abstractAims: Although Myroides spp. are primarily isolated from clinical sources, they have also been isolated from freshwater fish, stainless steel weldment and catheter biofilms. The ability of these nonmotile isolates to adhere to surfaces and the role of hydrophobicity and autoaggregation were investigated. Methods: Adherence of Myroides odoratus isolates from tilapia was investigated using microtitre adherence assays under varying environmental conditions. Hydrophobicity, aggregation and coaggregation were measured quantitatively. Biofilm structures were investigated using flow cells and microscopy. Results and Conclusions: Myroides odoratus isolates were strongly adherent in both nutrient-poor and -rich media at room temperature and nutrient-rich medium at 37°C. Isolates appeared hydrophilic, while autoaggregation indices ranged from 7% to 24·8%. Statistically significant positive correlations were observed between biofilm formation and autoaggregation. Strong coaggregation interactions were observed with several Gram-positive organisms, as well as with Listeria spp. In flow cells, M. odoratus formed an overlying carpet, with knitted chains of L. monocytogenes cells situated underneath attached to the perspex flow cell surface. Significance and Impact of Study: Myroides odoratus biofilm formation was influenced by temperature variation rather than altered nutrient levels. The association of Myroides spp. with Listeria spp. isolates suggests potential association in biofilm structures when these bacteria colonize biotic and/or abiotic surfaces. © Journal compilation © 2009 The Society for Applied Microbiology. No claim to original US Government works.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation107
dc.identifier.citation6
dc.identifier.issn13645072
dc.identifier.other10.1111/j.1365-2672.2009.04380.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11150
dc.subjectgenomic DNA
dc.subjectRNA 16S
dc.subjectbacterium
dc.subjectbioassay
dc.subjectbiofilm
dc.subjectcichlid
dc.subjectfish culture
dc.subjecthydrophobicity
dc.subjectnutrient availability
dc.subjectsteel
dc.subjectaquaculture
dc.subjectarticle
dc.subjectbacterial colonization
dc.subjectbacterium adherence
dc.subjectbacterium culture
dc.subjectbacterium isolation
dc.subjectbiofilm
dc.subjectcontrolled study
dc.subjectFlavobacteriaceae
dc.subjectfreshwater fish
dc.subjectGram negative bacterium
dc.subjecthydrophobicity
dc.subjectListeria monocytogenes
dc.subjectmicroscopy
dc.subjectMyroides
dc.subjectnonhuman
dc.subjectnutrient
dc.subjectpolymerase chain reaction
dc.subjectrandom amplified microsatellite
dc.subjectrestriction fragment length polymorphism
dc.subjecttemperature
dc.subjectTilapia
dc.subjectVibrio mimicus
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectBiofilms
dc.subjectFish Diseases
dc.subjectFlavobacteriaceae
dc.subjectFlavobacteriaceae Infections
dc.subjectHydrophobicity
dc.subjectStainless Steel
dc.subjectTemperature
dc.subjectTilapia
dc.subjectListeria
dc.subjectListeria monocytogenes
dc.subjectMyroides
dc.subjectMyroides odoratus
dc.subjectOreochromis mossambicus
dc.subjectPosibacteria
dc.subjectTilapia
dc.titleBiofilm-forming capacity, surface hydrophobicity and aggregation characteristics of Myroides odoratus isolated from South African Oreochromis mossambicus fish
dc.typeArticle
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