Characterization of triglycerides in vegetable oils by silver-ion packed-column supercritical fluid chromatography coupled to mass spectroscopy with atmospheric pressure chemical ionization and coordination ion spray

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dc.contributor.authorSandra P.
dc.contributor.authorMedvedovici A.
dc.contributor.authorZhao Y.
dc.contributor.authorDavid F.
dc.date.accessioned2011-05-15T15:59:36Z
dc.date.available2011-05-15T15:59:36Z
dc.date.issued2002
dc.description.abstractCharacterization of triglycerides in vegetable oils was achieved by silver-ion packed-column supercritical fluid chromatography (SI-pSFC) with mass spectrometric detection. Hyphenation was made using commercially available liquid chromatography-mass spectrometry (LC-MS) interfaces without any modification. A make-up fluid was delivered through a T-piece placed before or after the SFC restrictor by means of a high pressure pump. Atmospheric pressure chemical ionization (APCI) and coordination ion spray (CIS) with silver ions were used as ionization modes. Compared to UV detection, the sensitivity was increased by a factor of 100. Both ionization modes are generating similar structural information. Molecular ions [M-H]+ or [M-Ag+] are observed in the mass spectra with exception of the saturated triglycerides for which only CIS gives intense molecular ions. The position at which the fatty acids are esterified to the glycerol backbone can be elucidated by pSFC-APCI although it remains speculative whether this is valid for highly unsaturated triglycerides because reference compounds are not available to proof this. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationJournal of Chromatography A
dc.identifier.citation974
dc.identifier.citation02-Jan
dc.identifier.issn219673
dc.identifier.other10.1016/S0021-9673(02)01311-0
dc.identifier.urihttp://hdl.handle.net/10019.1/11263
dc.subjectAtmospheric pressure
dc.subjectEsterification
dc.subjectFatty acids
dc.subjectGlycerol
dc.subjectIonic conduction
dc.subjectLiquid chromatography
dc.subjectMass spectrometry
dc.subjectMolecular ions
dc.subjectVegetable oils
dc.subjectfatty acid
dc.subjectsilver
dc.subjecttriacylglycerol
dc.subjectvegetable oil
dc.subjectarticle
dc.subjectatmospheric pressure
dc.subjectesterification
dc.subjectionization
dc.subjectlipid analysis
dc.subjectliquid chromatography
dc.subjectmass spectrometry
dc.subjectpriority journal
dc.subjectsupercritical fluid chromatography
dc.subjectultraviolet radiation
dc.subjectchemistry
dc.subjectmethodology
dc.subjectAtmospheric Pressure
dc.subjectChromatography, Supercritical Fluid
dc.subjectMass Spectrometry
dc.subjectPlant Oils
dc.subjectTriglycerides
dc.titleCharacterization of triglycerides in vegetable oils by silver-ion packed-column supercritical fluid chromatography coupled to mass spectroscopy with atmospheric pressure chemical ionization and coordination ion spray
dc.typeArticle
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