Reciprocal chromosome painting among human, aardvark, and elephant (superorder Afrotheria) reveals the likely eutherian ancestral karyotype

Simple item page
dc.contributor.authorYang F.
dc.contributor.authorAlkalaeva E.Z.
dc.contributor.authorPerelman P.L.
dc.contributor.authorPardini A.T.
dc.contributor.authorHarrison W.R.
dc.contributor.authorO'Brien P.C.M.
dc.contributor.authorFu B.
dc.contributor.authorGraphodatsky A.S.
dc.contributor.authorFerguson-Smith M.A.
dc.contributor.authorRobinson T.J.
dc.date.accessioned2011-05-15T16:02:20Z
dc.date.available2011-05-15T16:02:20Z
dc.date.issued2003
dc.description.abstractThe Afrotheria, a supraordinal grouping of mammals whose radiation is rooted in Africa, is strongly supported by DNA sequence data but not by their disparate anatomical features. We have used flow-sorted human, aardvark, and African elephant chromosome painting probes and applied reciprocal painting schemes to representatives of two of the Afrotherian orders, the Tubulidentata (aardvark) and Proboscidea (elephants), in an attempt to shed additional light on the evolutionary affinities of this enigmatic group of mammals. Although we have not yet found any unique cytogenetic signatures that support the monophyly of the Afrotheria, embedded within the aardvark genome we find the strongest evidence yet of a mammalian ancestral karyotype comprising 2n = 44. This karyotype includes nine chromosomes that show complete conserved synteny to those of man, six that show conservation as single chromosome arms or blocks in the human karyotype but that occur on two different chromosomes in the ancestor, and seven neighbor-joining combinations (i.e., the synteny is maintained in the majority of species of the orders studied so far, but which corresponds to two chromosomes in humans). The comparative chromosome maps presented between human and these Afrotherian species provide further insight into mammalian genome organization and comparative genomic data for the Afrotheria, one of the four major evolutionary clades postulated for the Eutheria.
dc.description.versionArticle
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America
dc.identifier.citation100
dc.identifier.citation3
dc.identifier.issn278424
dc.identifier.other10.1073/pnas.0335540100
dc.identifier.urihttp://hdl.handle.net/10019.1/12421
dc.subjectAfrica
dc.subjectarticle
dc.subjectchromosome map
dc.subjectchromosome painting
dc.subjectDNA sequence
dc.subjectelephant
dc.subjectgenetic conservation
dc.subjectgenetic organization
dc.subjectgenome
dc.subjecthuman
dc.subjectkaryotype
dc.subjectmolecular evolution
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectAnimals
dc.subjectChromosome Banding
dc.subjectChromosome Painting
dc.subjectChromosomes
dc.subjectElephants
dc.subjectEvolution
dc.subjectEvolution, Molecular
dc.subjectGenome
dc.subjectGenome, Human
dc.subjectHumans
dc.subjectKaryotyping
dc.subjectPhylogeny
dc.subjectTime Factors
dc.subjectXenarthra
dc.subjectelephant
dc.subjectElephantidae
dc.subjectEutheria
dc.subjectLoxodonta
dc.subjectMammalia
dc.subjectTubulidentata
dc.titleReciprocal chromosome painting among human, aardvark, and elephant (superorder Afrotheria) reveals the likely eutherian ancestral karyotype
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications