Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse

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dc.contributor.authorMarx, Isa Jacoba
dc.contributor.authorVan Wyk, Niel
dc.contributor.authorSmit, Salome
dc.contributor.authorJacobson, Daniel
dc.contributor.authorViljoen-Bloom, Marinda
dc.contributor.authorVolschenk, Heinrich
dc.date.accessioned2014-04-14T07:48:38Z
dc.date.available2014-04-14T07:48:38Z
dc.date.issued2013-11
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at :en_ZA
dc.descriptionMarx, I. J. et al. 2013. Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnology for biofuels, 6:172, doi:10.1186/1754-6834-6-172.en_ZA
dc.descriptionThe original publication is available at http://www.biotechnologyforbiofuels.com/en_ZA
dc.description.abstractBackground: The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme profile of a newly isolated Trichoderma asperellum S4F8 strain with that of Trichoderma reesei Rut C30, cultured on sugarcane bagasse (SCB) using solid-state fermentation (SSF). Results: Comparison of the lignocellulolytic enzyme profiles of S4F8 and Rut C30 showed that S4F8 had significantly higher hemicellulase and β-glucosidase enzyme activities. Liquid chromatography tandem mass spectrometry analysis of the two fungal secretomes enabled the detection of 815 proteins in total, with 418 and 397 proteins being specific for S4F8 and Rut C30, respectively, and 174 proteins being common to both strains. In-depth analysis of the associated biological functions and the representation of glycoside hydrolase family members within the two secretomes indicated that the S4F8 secretome contained a higher diversity of main and side chain hemicellulases and β-glucosidases, and an increased abundance of some of these proteins compared with the Rut C30 secretome. Conclusions: In SCB SSF, T. asperellum S4F8 produced a more complex lignocellulolytic cocktail, with enhanced hemicellulose and cellobiose hydrolysis potential, compared with T. reesei Rut C30. This bodes well for the development of a more cost-effective and efficient lignocellulolytic enzyme cocktail from T. asperellum for lignocellulosic feedstock hydrolysis.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extent13 p. : ill.
dc.identifier.citationMarx, I. J. et al. 2013. Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnology for biofuels, 6:172, doi:10.1186/1754-6834-6-172.en_ZA
dc.identifier.issn1754-6834 (print)
dc.identifier.issn1754-6834 (online)
dc.identifier.otherdoi:10.1186/1754-6834-6-172
dc.identifier.urihttp://hdl.handle.net/10019.1/86169
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTrichoderma asperellum S4F8en_ZA
dc.subjectTrichoderma reesei Rut C30en_ZA
dc.subjectSecretomeen_ZA
dc.subjectSolid-state fermentationen_ZA
dc.subjectSugarcane bagasseen_ZA
dc.subjectProteomicsen_ZA
dc.titleComparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasseen_ZA
dc.typeArticleen_ZA
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