Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse
Date
2013-11
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Background: The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme profile of a newly isolated Trichoderma asperellum S4F8 strain with that of Trichoderma reesei Rut C30, cultured on sugarcane bagasse (SCB) using solid-state fermentation (SSF).
Results: Comparison of the lignocellulolytic enzyme profiles of S4F8 and Rut C30 showed that S4F8 had
significantly higher hemicellulase and β-glucosidase enzyme activities. Liquid chromatography tandem mass
spectrometry analysis of the two fungal secretomes enabled the detection of 815 proteins in total, with 418 and 397 proteins being specific for S4F8 and Rut C30, respectively, and 174 proteins being common to both strains. In-depth analysis of the associated biological functions and the representation of glycoside hydrolase family members within the two secretomes indicated that the S4F8 secretome contained a higher diversity of main and side chain hemicellulases and β-glucosidases, and an increased abundance of some of these proteins compared with the Rut C30 secretome.
Conclusions: In SCB SSF, T. asperellum S4F8 produced a more complex lignocellulolytic cocktail, with enhanced
hemicellulose and cellobiose hydrolysis potential, compared with T. reesei Rut C30. This bodes well for the
development of a more cost-effective and efficient lignocellulolytic enzyme cocktail from T. asperellum for
lignocellulosic feedstock hydrolysis.
Description
Publication of this article was funded by the Stellenbosch University Open Access Fund.
The original publication is available at :
Marx, I. J. et al. 2013. Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnology for biofuels, 6:172, doi:10.1186/1754-6834-6-172.
The original publication is available at http://www.biotechnologyforbiofuels.com/
The original publication is available at :
Marx, I. J. et al. 2013. Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnology for biofuels, 6:172, doi:10.1186/1754-6834-6-172.
The original publication is available at http://www.biotechnologyforbiofuels.com/
Keywords
Trichoderma asperellum S4F8, Trichoderma reesei Rut C30, Secretome, Solid-state fermentation, Sugarcane bagasse, Proteomics
Citation
Marx, I. J. et al. 2013. Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnology for biofuels, 6:172, doi:10.1186/1754-6834-6-172.