Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR
dc.contributor.author | Ramiah K. | |
dc.contributor.author | van Reenen C.A. | |
dc.contributor.author | Dicks L.M.T. | |
dc.date.accessioned | 2011-05-15T15:56:47Z | |
dc.date.available | 2011-05-15T15:56:47Z | |
dc.date.issued | 2007 | |
dc.description.abstract | Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA by Lactobacillus plantarum 423, grown in the presence of bile, pancreatin and at low pH, was studied by real-time PCR. Mub, MapA and EF-Tu were up-regulated in the presence of mucus, proportional to increasing concentrations. Expression of MapA was up-regulated in the presence of 3.0 g/l bile and 3.0 g/l pancreatin at pH 6.5. Similar results were recorded in the presence of 10.0 g/l bile and 10.0 g/l pancreatin at pH 6.5. Expression of Mub was down-regulated in the presence of bile and pancreatin, whilst the expression of EF-Tu and plaA remained unchanged. Expression of Mub and MapA remained unchanged at pH 4.0, whilst expression of EF-Tu and plaA were up-regulated. Expression of MapA was down-regulated in the presence of 1.0 g/l l-cysteine HCl, suggesting that the gene is regulated by transcription attenuation that involves cysteine. © 2007 Elsevier B.V. All rights reserved. | |
dc.description.version | Article | |
dc.identifier.citation | International Journal of Food Microbiology | |
dc.identifier.citation | 116 | |
dc.identifier.citation | 3 | |
dc.identifier.issn | 1681605 | |
dc.identifier.other | 10.1016/j.ijfoodmicro.2007.02.011 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/10041 | |
dc.subject | bacteriocin | |
dc.subject | cysteine | |
dc.subject | hydrochloric acid | |
dc.subject | pancreatin | |
dc.subject | article | |
dc.subject | bile | |
dc.subject | controlled study | |
dc.subject | down regulation | |
dc.subject | gene control | |
dc.subject | gene expression | |
dc.subject | genetic transcription | |
dc.subject | Lactobacillus plantarum | |
dc.subject | mucus | |
dc.subject | nonhuman | |
dc.subject | nucleotide sequence | |
dc.subject | pH | |
dc.subject | real time polymerase chain reaction | |
dc.subject | upregulation | |
dc.subject | Bacterial Adhesion | |
dc.subject | Bacterial Proteins | |
dc.subject | Bile | |
dc.subject | Gene Expression Regulation, Bacterial | |
dc.subject | Humans | |
dc.subject | Hydrogen-Ion Concentration | |
dc.subject | Lactobacillus plantarum | |
dc.subject | Mucus | |
dc.subject | Pancreatin | |
dc.subject | Polymerase Chain Reaction | |
dc.subject | Probiotics | |
dc.subject | Lactobacillus plantarum | |
dc.title | Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR | |
dc.type | Article |