Analysis of the HMW glutenin subunit composition of wheat cultivars by SDS-PAGE
dc.contributor.author | De Villiers O.T. | |
dc.contributor.author | Bosman M. | |
dc.date.accessioned | 2011-05-15T15:55:08Z | |
dc.date.available | 2011-05-15T15:55:08Z | |
dc.date.issued | 1996 | |
dc.description.abstract | The composition of the high-molecular weight (HMW) glutenin subunits of 13 wheat cultivars grown in the Western Cape was determined using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). The molecular weight of the different subunits was determined using standard proteins with known molecular weights. The Glu-1 quality score and the frequency of the subunits controlled by chromosomes 1A, 1B and 1D were calculated for the different cultivars. Ten different patterns of HMW glutenin subunits consisting of 4-5 protein bands were found. A total of 14 different HMW glutenin subunits were found, enabling most of the cultivars to be identified. | |
dc.description.version | Article | |
dc.identifier.citation | South African Journal of Plant and Soil | |
dc.identifier.citation | 13 | |
dc.identifier.citation | 1 | |
dc.identifier.issn | 2571862 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/9603 | |
dc.subject | Triticum aestivum | |
dc.title | Analysis of the HMW glutenin subunit composition of wheat cultivars by SDS-PAGE | |
dc.type | Article |