Modification of the QuantiFERON-TB Gold (In-Tube) assay for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)

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dc.contributor.authorParsons S.D.C.
dc.contributor.authorCooper D.
dc.contributor.authorMcCall A.J.
dc.contributor.authorMcCall W.A.
dc.contributor.authorStreicher E.M.
dc.contributor.authorle Maitre N.C.
dc.contributor.authorMuller A.
dc.contributor.authorGey van Pittius N.C.
dc.contributor.authorWarren R.M.
dc.contributor.authorvan Helden P.D.
dc.date.accessioned2011-10-13T16:59:04Z
dc.date.available2011-10-13T16:59:04Z
dc.date.issued2011
dc.description.abstractAfrican buffaloes (Syncerus caffer) are the most significant wildlife maintenance hosts of Mycobacterium bovis, the causative organism of bovine tuberculosis (BTB). Current diagnostic tests for the detection of M. bovis infection in free-ranging buffaloes have numerous limitations and we wished to evaluate a modification to a human TB assay, the QuantiFERON-TB Gold (In-Tube) assay (QFT), as a practical diagnostic test for BTB in buffaloes. One hundred and seventy-five buffaloes were tested using the single intradermal comparative tuberculin test (SICTT) and a modified QFT (mQFT). An appropriate cut-off point for the mQFT was derived from SICTT results using receiver operator characteristic curve analysis. Twenty-six SICTT-positive buffaloes were killed and subjected to necropsy, and selected tissues were processed for mycobacterial culture and speciation. An optimal cut-off point for the mQFT was calculated as 66. pg/ml. The assay correctly detected 39/40 SICTT-positive buffaloes and 129/134 TST-negative buffaloes and M. bovis was cultured from 21/26 slaughtered SICTT/mQFT-positive animals. The mQFT shows promise as a practical test for M. bovis infection in buffaloes and shows a sensitivity and specificity at least similar to that of the TST. © 2011 Elsevier B.V.
dc.description.versionArticle
dc.identifier.citationVeterinary Immunology and Immunopathology
dc.identifier.citation142
dc.identifier.citation02-Jan
dc.identifier.citationhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79957951221&partnerID=40&md5=f5747f36cd9afc5a8f614f5c270df512
dc.identifier.issn1652427
dc.identifier.other10.1016/j.vetimm.2011.04.006
dc.identifier.urihttp://hdl.handle.net/10019.1/16970
dc.titleModification of the QuantiFERON-TB Gold (In-Tube) assay for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer)
dc.typeArticle
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