Characteristics of Fps1-dependent and -independent glycerol transport in Saccharomyces cerevisiae

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dc.contributor.authorSutherland F.C.W.
dc.contributor.authorLages F.
dc.contributor.authorLucas C.
dc.contributor.authorLuyten K.
dc.contributor.authorAlbertyn J.
dc.contributor.authorHohmann S.
dc.contributor.authorPrior B.A.
dc.contributor.authorKilian S.G.
dc.date.accessioned2011-05-15T16:01:53Z
dc.date.available2011-05-15T16:01:53Z
dc.date.issued1997
dc.description.abstractEadie-Hofstee plots of glycerol uptake in wild-type Saccharomyces cerevisiae W303-1A grown on glucose showed the presence of both saturable transport and simple diffusion, whereas an fps1Δ mutant displayed only simple diffusion. Transformation of the fps1Δ mutant with the glpF gene, which encodes glycerol transport in Escherichia coli, restored biphasic transport kinetics. Yeast extract-peptone-dextrose-grown wild-type cells had a higher passive diffusion constant than the fps1Δ mutant, and ethanol enhanced the rate of proton diffusion to a greater extent in the wild type than in the fps1Δ mutant. In addition, the lipid fraction of the fps1Δ mutant contained a lower percentage of phospholipids and a higher percentage of glycolipids than that of the wild type. Fps1p, therefore, may be involved in the regulation of lipid metabolism in S. cerevisiae, affecting membrane permeability in addition to fulfilling its specific role in glycerol transport. Simultaneous uptake of glycerol and protons occurred in both glycerol- and ethanol-grown wild-type and fps1Δ cells and resulted in the accumulation of glycerol at an inside-to-outside ratio of 12:1 to 15:1. Carbonyl cyanide m-chlorophenylhydrazone prevented glycerol accumulation in both strains and abolished transport in the fps1Δ mutant grown on ethanol. Likewise, 2,4-dinitrophenol inhibited transport in glycerol-grown wild-type cells. These results indicate the presence of an Fps1p-dependent facilitated diffusion system in glucose-grown cells and an Fps1p-independent proton symport system in derepressed cells.
dc.description.versionArticle
dc.identifier.citationJournal of Bacteriology
dc.identifier.citation179
dc.identifier.citation24
dc.identifier.issn219193
dc.identifier.urihttp://hdl.handle.net/10019.1/12205
dc.subjectglycerol
dc.subjectarticle
dc.subjectdiffusion
dc.subjectgene deletion
dc.subjectintracellular transport
dc.subjectlipid composition
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectsaccharomyces cerevisiae
dc.subjecttransport kinetics
dc.subjectAquaporins
dc.subjectBacterial Outer Membrane Proteins
dc.subjectBiological Transport
dc.subjectDiffusion
dc.subjectEscherichia coli Proteins
dc.subjectEthanol
dc.subjectFungal Proteins
dc.subjectGlycerol
dc.subjectLipids
dc.subjectMembrane Proteins
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectEscherichia coli
dc.subjectSaccharomyces cerevisiae
dc.titleCharacteristics of Fps1-dependent and -independent glycerol transport in Saccharomyces cerevisiae
dc.typeArticle
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