Characterisation of South African isolates of Fusarium oxysporum f.sp. cubense from Cavendish bananas

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dc.contributor.authorVisser, Marinda
dc.contributor.authorGordon, Tom
dc.contributor.authorFourie, Gerda
dc.contributor.authorViljoen, Altus
dc.date.accessioned2011-05-15T15:55:10Z
dc.date.available2011-05-15T15:55:10Z
dc.date.issued2010-04
dc.descriptionThe original publication is available at http://www.sajs.co.za/en_ZA
dc.description.abstractFusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f.sp. cubense (Foc), is a serious vascular disease of bananas in most subtropical and tropical regions of the world. Twenty-four vegetative compatibility groups (VCGs) and three pathogenic races have been identified in Foc, reflecting a relatively high genetic diversity for an asexual fungus. To characterise a South African population of Foc, a collection of 128 isolates from diverse geographic origins were isolated from diseased Cavendish bananas and subjected to VCG analysis and sequencing of the translation elongation factor 1-α (TEF) gene region. The presence of mating type genes was also determined using MAT-1 and MAT-2 specific primers. VCG 0120 was established as the only VCG of Foc present in the South African population studied. Only the MAT-2 idiomorph was present in all the local isolates of Foc. A phylogenetic analysis of DNA sequences of the TEF gene region revealed that the South African isolates grouped closely with VCG 0120 isolates from Australia and Asia. These results suggest that the South African population of Foc was most likely introduced in a limited number of events and that it had spread with infected planting material within the country. The presence of only one mating type and the limited diversity in this pathogen render it unlikely to rapidly overcome disease management strategies involving host resistance. © 2010. The Authors.en_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extent6 p.
dc.identifier.citationVisser, M., Gordon, T., Fourie, G. & Viljoen, A. 2010. Characterisation of South African isolates of Fusarium oxysporum f.sp. cubense from Cavendish bananas. South African Journal of Science, 106(3/4), doi:10.4102/sajs.v106i3/4.154en_ZA
dc.identifier.issn1996-7489 (online)
dc.identifier.issn0038-2353 (print)
dc.identifier.otherdoi:10.4102/sajs.v106i3/4.154
dc.identifier.urihttp://hdl.handle.net/10019.1/9616
dc.publisherAcademy of Science of South Africa (ASSAf)en_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectCavendish bananas -- Disease controlen_ZA
dc.subjectFusarium oxysporum f.sp. cubense (Foc)en_ZA
dc.subjectFusarium wilten_ZA
dc.subjectTranslation elongation factor 1-α (TEF) gene region (TEF) -- Phylogenetics analysisen_ZA
dc.titleCharacterisation of South African isolates of Fusarium oxysporum f.sp. cubense from Cavendish bananasen_ZA
dc.typeArticle
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