Efficiency of the pTF-FC2 pas Poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the Lon protease

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dc.contributor.authorSmith A.S.G.
dc.contributor.authorRawlings D.E.
dc.date.accessioned2011-05-15T16:01:53Z
dc.date.available2011-05-15T16:01:53Z
dc.date.issued1998
dc.description.abstractThe stabilization of a test plasmid by the proteic, poison-antidote plasmid addiction system (pas) of plasmid pTF-FC2 was host strain dependent, with a 100-fold increase in stability in Escherichia coli CSH50, a 2.5-fold increase in E. coli JM105, and no detectable stabilization in E. coli strains JM107 and JM109. The lethality of the PasB toxin was far higher in the E. coli strains in which the pas was most effective. Models for the way in which poison-antidote systems stabilize plasmids require that the antidote have a much higher rate of turnover than that of the toxin. A decrease in host cell death following plasmid loss from an E. coli lon mutant and a decrease in plasmid stability suggested that the Lon protease plays a role in the rate of turnover of PasA antidote.
dc.description.versionArticle
dc.identifier.citationJournal of Bacteriology
dc.identifier.citation180
dc.identifier.citation20
dc.identifier.issn219193
dc.identifier.urihttp://hdl.handle.net/10019.1/12203
dc.subjectarticle
dc.subjectbacterium mutant
dc.subjectenzyme activity
dc.subjectescherichia coli
dc.subjectgene expression
dc.subjectgene overexpression
dc.subjectgene structure
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectplasmid
dc.subjectpriority journal
dc.subjectAntidotes
dc.subjectATP-Dependent Proteases
dc.subjectBacterial Proteins
dc.subjectBacterial Toxins
dc.subjectDNA Replication
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectHeat-Shock Proteins
dc.subjectPlasmids
dc.subjectProtease La
dc.subjectSerine Endopeptidases
dc.subjectSpecies Specificity
dc.subjectBacteria (microorganisms)
dc.subjectEscherichia coli
dc.subjectNegibacteria
dc.titleEfficiency of the pTF-FC2 pas Poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the Lon protease
dc.typeArticle
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