The osmotic stress tolerance of basidiomycetous yeasts: RESEARCH ARTICLE

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dc.contributor.authorTekolo O.M.
dc.contributor.authorMcKenzie J.
dc.contributor.authorBotha A.
dc.contributor.authorPrior B.A.
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2010
dc.description.abstractThe growth and accumulation of intracellular polyols at reduced water activity (aw) were studied in 40 basidiomycetous yeast strains. The growth of most strains showed greater tolerance to NaCl than sorbitol at the same aw. No strain was able to grow below 0.90aw. 13C nuclear magnetic resonance spectroscopy revealed that glycerol was the major solute accumulated intracellularly by all the yeasts when grown to 0.96aw (NaCl). Arabitol or mannitol was also accumulated in some yeasts, whereas a few only accumulated glycerol. Analysis of six yeasts in detail revealed that the intracellular glycerol concentrations of five yeasts increased significantly when grown at 0.96aw (NaCl or sorbitol) compared with growth at 0.998aw. Arabitol and mannitol concentrations also increased, but not to the same degree. Intracellular potassium concentrations decreased when grown at 0.96aw (NaCl or sorbitol) and sodium increased, but only when grown at 0.96aw (NaCl). The survival of nine strains was evaluated in soil cultures and it was found that all grew at 100% field capacity, whereas at lower field capacity, only some strains grew or survived. The growth of basidiomycetous yeasts appears to be more sensitive to reduced aw than ascomycetous yeasts. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation10
dc.identifier.citation4
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2010.00612.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12616
dc.subjectglycerol
dc.subjectmannitol
dc.subjectpolyol
dc.subjectpotassium
dc.subjectsodium chloride
dc.subjectsorbitol
dc.subjectarticle
dc.subjectBasidiomycetes
dc.subjectbioaccumulation
dc.subjectcarbon nuclear magnetic resonance
dc.subjectcell culture
dc.subjectcell growth
dc.subjectcell survival
dc.subjectCryptococcus laurentii
dc.subjectcryptococcus macerans
dc.subjectCryptococcus neoformans
dc.subjectDioszegia hungarica
dc.subjectFilobasidiella
dc.subjectfilobasidiella neoformans
dc.subjectFilobasidium capsuligenum
dc.subjectFilobasidium floriforme
dc.subjectnonhuman
dc.subjectosmotic stress
dc.subjectpotassium cell level
dc.subjectRhodotorula minuta
dc.subjectRhodotorula mucilaginosa
dc.subjectsoil
dc.subjectSporidiobolus salmonicolor
dc.subjectSterigmatomyces halophilus
dc.subjectyeast cell
dc.subjectBasidiomycota
dc.subjectCytosol
dc.subjectGlycerol
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectMannitol
dc.subjectOsmotic Pressure
dc.subjectPotassium
dc.subjectSodium Chloride
dc.subjectSorbitol
dc.subjectStress, Physiological
dc.subjectSugar Alcohols
dc.titleThe osmotic stress tolerance of basidiomycetous yeasts: RESEARCH ARTICLE
dc.typeArticle
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