Degradation of proteins and starch by combined immobilization of protease, α-amylase and β-galactosidase on a single electrospun nanofibrous membrane

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dc.contributor.authorCloete, William J.en_ZA
dc.contributor.authorHayward, Stefanen_ZA
dc.contributor.authorSwart, Pieteren_ZA
dc.contributor.authorKlumperman, Berten_ZA
dc.date.accessioned2021-10-12T13:44:29Z
dc.date.available2021-10-12T13:44:29Z
dc.date.issued2019-01-31
dc.descriptionCITATION: Cloete W. J. et al. 2019. Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane. Molecules, 24(3):508. doi:10.3390/molecules2403050
dc.descriptionThe original publication is available at https://www.mdpi.com/journal/molecules
dc.description.abstractTwo commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone.en_ZA
dc.description.sponsorshipDepartment of Science and Technology
dc.description.sponsorshipNational Research Foundation (NRF)
dc.description.urihttps://www.mdpi.com/1420-3049/24/3/508
dc.description.versionPublisher’s version
dc.format.extent9 pages : illustrations (some color)en_ZA
dc.identifier.citationCloete W. J. et al. 2019. Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane. Molecules, 24(3):508. doi:10.3390/molecules2403050
dc.identifier.issn1420-3049 (online)
dc.identifier.otherdoi:10.3390/molecules24030508
dc.identifier.urihttp://hdl.handle.net/10019.1/123214
dc.language.isoen_ZAen_ZA
dc.publisherMDPI
dc.rights.holderAuthors retain rights
dc.subjectPoly(styrene-co-maleic anhydride)en_ZA
dc.subjectEnzymes -- Biotechnologyen_ZA
dc.subjectImmobilizationen_ZA
dc.subjectProteaseen_ZA
dc.subjectProteolytic enzymesen_ZA
dc.subjectImmobilized enzymes -- Biotechnologyen_ZA
dc.subjectProteins -- Degradationen_ZA
dc.subjectStarch -- Synthesisen_ZA
dc.titleDegradation of proteins and starch by combined immobilization of protease, α-amylase and β-galactosidase on a single electrospun nanofibrous membraneen_ZA
dc.typeArticleen_ZA
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