Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae

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dc.contributor.authorVan Reenen C.A.
dc.contributor.authorChikindas M.L.
dc.contributor.authorVan Zyl W.H.
dc.contributor.authorDicks L.M.T.
dc.date.accessioned2011-05-15T15:56:49Z
dc.date.available2011-05-15T15:56:49Z
dc.date.issued2003
dc.description.abstractLactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. This protein is bactericidal for many Gram-positive foodborne pathogens and spoilage bacteria, including Listeria spp., Staphylococcus spp., Pediococcus spp., Lactobacillus spp., etc. The DNA sequence of the plantaricin 423-encoding region on plasmid pPLA4 revealed a four open reading frame (ORF) operon structure similar to pediocin PA-1/AcH from Pediococcus acidilactici and coagulin from Bacillus coagulans I4. The first ORF, plaA, encodes a 56-amino acid prepeptide consisting of a 37-amino acid mature molecule, with a 19-amino acid N-terminal leader peptide. The second ORF, plaB, encodes a putative immunity protein with protein sequence similarities to several bacteriocin immunity proteins. The plaC and plaD genes are virtually identical to pedC and pedD of the pediocin PA-1 operon, as well as coaC and coaD of the coagulin operon. Plantaricin 423 was cloned on a shuttle vector under the control of a yeast promoter and heterologously produced in Saccharomyces cerevisiae. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationInternational Journal of Food Microbiology
dc.identifier.citation81
dc.identifier.citation1
dc.identifier.issn1681605
dc.identifier.other10.1016/S0168-1605(02)00164-2
dc.identifier.urihttp://hdl.handle.net/10019.1/10056
dc.subjectamino acid
dc.subjectbacterial protein
dc.subjectbacteriocin
dc.subjectcoagulin
dc.subjectfood preservative
dc.subjectpediocin
dc.subjectpeptide
dc.subjectplantaricin
dc.subjectunclassified drug
dc.subjectamino acid sequence
dc.subjectamino terminal sequence
dc.subjectarticle
dc.subjectBacillus coagulans
dc.subjectbacterial gene
dc.subjectbacterial strain
dc.subjectbacterium
dc.subjectcontrolled study
dc.subjectDNA sequence
dc.subjectLactobacillus plantarum
dc.subjectmolecular cloning
dc.subjectmolecule
dc.subjectnonhuman
dc.subjectopen reading frame
dc.subjectoperon
dc.subjectpedicoccus acidilacti
dc.subjectplasmid
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectSaccharomyces cerevisiae
dc.subjectsequence homology
dc.subjectshuttle vector
dc.subjectAmino Acid Sequence
dc.subjectBacteriocins
dc.subjectBase Sequence
dc.subjectDNA, Bacterial
dc.subjectLactobacillus
dc.subjectMolecular Sequence Data
dc.subjectOpen Reading Frames
dc.subjectPlasmids
dc.subjectSaccharomyces cerevisiae
dc.subjectSpectrometry, Mass, Electrospray Ionization
dc.subjectBacillus coagulans
dc.subjectLactobacillus
dc.subjectLactobacillus plantarum
dc.subjectListeria
dc.subjectPediococcus
dc.subjectPediococcus acidilactici
dc.subjectPosibacteria
dc.subjectSaccharomyces
dc.subjectSaccharomyces cerevisiae
dc.subjectStaphylococcus
dc.subjectunidentified shuttle vector
dc.titleCharacterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae
dc.typeArticle
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