Digestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymes

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dc.contributor.authorVan de Vyver, W. F. J.en_ZA
dc.contributor.authorUseni, B. A.en_ZA
dc.date.accessioned2013-07-03T08:19:44Z
dc.date.available2013-07-03T08:19:44Z
dc.date.issued2012
dc.descriptionCITATION: Van de Vyver, W. F. J. & Useni, B. A. 2012. Digestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymes. South African Journal of Animal Science, 42(4):488-492, doi:10.4314/sajas.v42i5.9.
dc.descriptionThe original publication is available at http://www.sasas.co.za
dc.description.abstractThe aim of the study was to determine the effect of an exogenous fibrolytic enzyme (EFE) on the ruminal disappearance of dry matter (DM), neutral detergent fibre (NDF), crude protein (CP) and on the microbial protein synthesis (MPS) in sheep of a milled substrate consisting of a 50 : 50 mixture of lucerne hay (LH) and wheat straw (WS). Four cannulated Döhne-Merino sheep were randomly assigned to two groups in a 2 x 2 cross-over experiment. Additional to the in situ study, the effects of an exogenous fibrolytic enzyme (EFE) on the in vitro gas production (GP) and ANKOM digestion systems on the mixture of milled LH and WS were determined. The substrate was pre-treated with distilled water (control) or EFE (treatment) 12 hours prior to incubation to allow interaction between the substrate and enzyme. Adding EFE significantly improved the in vitro cumulative gas production (GP), but it did not affect the in vitro MPS measured as purine derivates of the GP residues. The enzyme significantly increased the in vitro DM and NDF disappearances after 36 hours. However, the in situ disappearances of DM, NDF and CP of the roughage mixture were not affected by the EFE. Further, the in situ MPS was increased significantly with the EFE treatment of the mixed substrate. It is concluded that the improved ruminal digestion of DM, NDF and CP is not only due to direct enzyme hydrolysis, but also due to the improvement of MPS.
dc.description.urihttp://www.sasas.co.za/digestion-and-microbial-protein-synthesis-sheep-affected-exogenous-fibrolytic-enzymes
dc.description.versionPublisher's version
dc.format.extent5 pages
dc.identifier.citationSouth African Journal of Animal Science
dc.identifier.citationVan de Vyver, W. F. J. & Useni, B. A. 2012. Digestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymes. South African Journal of Animal Science, 42(4):488-492, doi:10.4314/sajas.v42i5.9.
dc.identifier.issn2221-4062 (online)
dc.identifier.issn0375-1589 (print)
dc.identifier.otherdoi:10.4314/sajas.v42i5.9
dc.identifier.urihttp://hdl.handle.net/10019.1/82614
dc.language.isoen
dc.publisherSouth African Society for Animal Science
dc.rights.holderAuthors retain copyright
dc.subjectSheepen_ZA
dc.subjectRuminantsen_ZA
dc.subjectRuminants -- Digestive organsen_ZA
dc.subjectProteins in animal nutritionen_ZA
dc.titleDigestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymesen_ZA
dc.typeArticle
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