A proteomic analysis of the ventral hippocampus of rats subjected to maternal separation and escitalopram treatment

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dc.contributor.authorMarais L.
dc.contributor.authorHattingh S.M.
dc.contributor.authorStein D.J.
dc.contributor.authorDaniels W.M.U.
dc.date.accessioned2011-05-15T15:59:57Z
dc.date.available2011-05-15T15:59:57Z
dc.date.issued2009
dc.description.abstractEarly life stress is known to predispose humans to the development of depression. Developmental stress has been shown to cause various changes in neurotransmitter systems, neurotrophin expression and the hypothalamic pituitary adrenal-axis in the rat brain. The aim of this study was to identify which cytosolic proteins are altered by maternal separation, as a model for depression, as well as by chronic antidepressant treatment. Rats were maternally separated from postnatal day 2-14 for 3 h per day while control rats were normally reared. Both groups were divided and received either escitalopram or saline injections for 6 weeks starting from postnatal day 40. The ventral hippocampal tissue was fractionated and the cytosolic fraction used for 2-D-gel electrophoresis and liquid chromatography coupled to mass spectrometry analyses to identify peptides. Mascot database searches were done to identify proteins that were differentially expressed between the groups. Proteins that were significantly changed by maternal separation included amongst others: molecular chaperones and proteins related to energy metabolism; neuroplasticity; oxidative stress regulation; and protein metabolism. Treatment with escitalopram, a selective-serotonin reuptake inhibitor, induced changes in a different group of proteins, except for a few involved in energy metabolism and neuroprotective pathways. The results indicate which cytosolic proteins are changed by early life stress and may therefore be involved in the development of depression. © 2009 Springer Science+Business Media, LLC.
dc.description.versionArticle
dc.identifier.citationMetabolic Brain Disease
dc.identifier.citation24
dc.identifier.citation4
dc.identifier.issn8857490
dc.identifier.other10.1007/s11011-009-9156-3
dc.identifier.urihttp://hdl.handle.net/10019.1/11453
dc.subjectamino acid
dc.subjectcell protein
dc.subjectcorticotropin
dc.subjectcytosolic protein
dc.subjectescitalopram
dc.subjectneurotrophin
dc.subjectserotonin uptake inhibitor
dc.subjectunclassified drug
dc.subjectamino acid sequence
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectcorticotropin release
dc.subjectdepression
dc.subjectdisease predisposition
dc.subjectdrug effect
dc.subjectelectrospray mass spectrometry
dc.subjectenergy metabolism
dc.subjectexperimental model
dc.subjecthippocampus
dc.subjecthypothalamus hypophysis adrenal system
dc.subjectlife stress
dc.subjectliquid chromatography
dc.subjectlong term care
dc.subjectmaternal deprivation
dc.subjectmother child relation
dc.subjectneurotransmission
dc.subjectnewborn
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectoxidative stress
dc.subjectperinatal period
dc.subjectprotein expression
dc.subjectproteomics
dc.subjectpsychopharmacotherapy
dc.subjectrat
dc.subjecttwo dimensional gel electrophoresis
dc.subjectunindexed sequence
dc.subjectAnimals
dc.subjectCausality
dc.subjectCitalopram
dc.subjectDepressive Disorder
dc.subjectDisease Models, Animal
dc.subjectEnergy Metabolism
dc.subjectFemale
dc.subjectHippocampus
dc.subjectMale
dc.subjectMaternal Deprivation
dc.subjectMolecular Chaperones
dc.subjectNerve Tissue Proteins
dc.subjectNeuronal Plasticity
dc.subjectOxidative Stress
dc.subjectProteomics
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectSerotonin Uptake Inhibitors
dc.subjectStress, Psychological
dc.subjectTime
dc.subjectTreatment Outcome
dc.subjectRattus
dc.titleA proteomic analysis of the ventral hippocampus of rats subjected to maternal separation and escitalopram treatment
dc.typeArticle
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