Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

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dc.contributor.authorSalma, Mohammaden_ZA
dc.contributor.authorRousseaux, Sandrineen_ZA
dc.contributor.authorSequeira-Le Grand, Anabelleen_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.contributor.authorAlexandre, Herveen_ZA
dc.date.accessioned2014-07-07T12:27:57Z
dc.date.available2014-07-07T12:27:57Z
dc.date.issued2013-10-29
dc.descriptionCITATION: Salma, M. et al. 2013. Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae. PLoS ONE, 8(10):e77600, doi:10.1371/journal.pone.0077600.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractThe Viable But Non Culturable (VBNC) state has been thoroughly studied in bacteria. In contrast, it has received much less attention in other microorganisms. However, it has been suggested that various yeast species occurring in wine may enter in VBNC following sulfite stress.In order to provide conclusive evidences for the existence of a VBNC state in yeast, the ability of Saccharomyces cerevisiae to enter into a VBNC state by applying sulfite stress was investigated. Viable populations were monitored by flow cytometry while culturable populations were followed by plating on culture medium. Twenty-four hours after the application of the stress, the comparison between the culturable population and the viable population demonstrated the presence of viable cells that were non culturable. In addition, removal of the stress by increasing the pH of the medium at different time intervals into the VBNC state allowed the VBNC S. cerevisiae cells to “resuscitate”. The similarity between the cell cycle profiles of VBNC cells and cells exiting the VBNC state together with the generation rate of cells exiting VBNC state demonstrated the absence of cellular multiplication during the exit from the VBNC state. This provides evidence of a true VBNC state. To get further insight into the molecular mechanism pertaining to the VBNC state, we studied the involvement of the SSU1 gene, encoding a sulfite pump in S. cerevisiae. The physiological behavior of wild-type S. cerevisiae was compared to those of a recombinant strain overexpressing SSU1 and null Δssu1 mutant. Our results demonstrated that the SSU1 gene is only implicated in the first stages of sulfite resistance but not per se in the VBNC phenotype. Our study clearly demonstrated the existence of an SO2-induced VBNC state in S. cerevisiae and that the stress removal allows the “resuscitation” of VBNC cells during the VBNC state.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077600
dc.description.versionPublisher's version
dc.format.extent11 pages
dc.identifier.citationSalma, M. et al. 2013. Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae. PLoS ONE, 8(10):e77600, doi:10.1371/journal.pone.0077600.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0077600
dc.identifier.urihttp://hdl.handle.net/10019.1/93881
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectYeasten_ZA
dc.subjectViable But Non Culturable Stateen_ZA
dc.subjectYeast fungi -- Biotechnologyen_ZA
dc.subjectWine and wine making -- Microbiologyen_ZA
dc.titleCharacterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiaeen_ZA
dc.typeArticle
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