Optimization of a method for the extraction and quantification of carotenoids and chlorophylls during ripening in grape berries (Vitis vinifera cv. Merlot)

Simple item page
dc.contributor.authorKamffer Z.
dc.contributor.authorBindon K.A.
dc.contributor.authorOberholster A.
dc.date.accessioned2011-05-15T15:56:50Z
dc.date.available2011-05-15T15:56:50Z
dc.date.issued2010
dc.description.abstractAn extraction method to identify and quantify the carotenoid and chlorophyll profile of lyophilized tissue from unripe (green) to ripe (red) Merlot grape berries was developed. The RP-HPLC method baseline separated all of the carotenoids and chlorophylls and their derivatives. Problems encountered during sample storage and extraction are discussed as well as possible alternative methods. This study confirmed that carotenoids and chlorophylls decreased on a per berry (μg/berry) and concentration (μg/g) basis from veraison to harvest over two growing seasons. The carotenoid 5,8-epoxy-β-carotene was quantified for the first time in grapes and represents a significant amount of the total carotenoids present at harvest. All the carotenoids and chlorophylls except β-carotene appeared to be sensitive to seasonal variation in climatic conditions. Lutein and β-carotene were found to be the most abundant carotenoids present in Merlot grape berries together with chlorophyll a for both seasons studied. © 2010 American Chemical Society.
dc.description.versionArticle
dc.identifier.citationJournal of Agricultural and Food Chemistry
dc.identifier.citation58
dc.identifier.citation11
dc.identifier.issn218561
dc.identifier.other10.1021/jf1004308
dc.identifier.urihttp://hdl.handle.net/10019.1/10063
dc.subjectcarotenoid
dc.subjectchlorophyll
dc.subjectplant extract
dc.subjectarticle
dc.subjectchemistry
dc.subjectevaluation
dc.subjectfruit
dc.subjectgrape
dc.subjecthigh performance liquid chromatography
dc.subjectmethodology
dc.subjectphysiology
dc.subjectseason
dc.subjectCarotenoids
dc.subjectChlorophyll
dc.subjectChromatography, High Pressure Liquid
dc.subjectFruit
dc.subjectPlant Extracts
dc.subjectSeasons
dc.subjectVitis
dc.subjectVitaceae
dc.subjectVitis vinifera
dc.titleOptimization of a method for the extraction and quantification of carotenoids and chlorophylls during ripening in grape berries (Vitis vinifera cv. Merlot)
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Publications